Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Labeling DNA Probes

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

A Network Pharmacology Study on the Active Ingredients and Potential Targets of <i>Tripterygium wilfordii</i> Hook for Treatment of Rheumatoid Arthritis.

Evidence-based complementary and alternative medicine : eCAM·2019

Same author

A PAH-degrading bacterial community enriched with contaminated agricultural soil and its utility for microbial bioremediation.

Environmental pollution (Barking, Essex : 1987)·2019

Same author

Size-Dependent Relaxation Processes of Photoexcited [ n]Cycloparaphenylenes ( n = 5-12): Significant Contribution of Internal Conversion in Smaller Rings.

The journal of physical chemistry. A·2019

Same author

NfiR, a New Regulatory Noncoding RNA (ncRNA), Is Required in Concert with the NfiS ncRNA for Optimal Expression of Nitrogenase Genes in Pseudomonas stutzeri A1501.

Applied and environmental microbiology·2019

Same author

A novel homolateral and dicationic AIEgen for the sensitive detection of casein.

The Analyst·2019

Same author

Terahertz Oscilloscope for Recording Time Information of Ultrashort Electron Beams.

Physical review letters·2019

Same journal

Advances in application of microplasmas for non-metallic species analysis by optical spectrometry.

The Analyst·2026

Same journal

Sulfur vacancy-mediated self-photocatalysis-boosted electrochemiluminescence sensing <i>via</i> molecular oxygen activation for sensitive detection of isocarbophos.

The Analyst·2026

Same journal

Analytical challenges in mapping the subcellular metabolome and lipidome.

The Analyst·2026

Same journal

Threshold-guided multiplex PCR-LFA: a step toward UTI pathogen detection.

The Analyst·2026

Same journal

Aptamer-based CRISPR-Cas12a fluorescent biosensors for serum biomarker detection.

The Analyst·2026

Same journal

A two-step centrifugal microfluidic platform for semi-automated IGRA detection of tuberculosis based on chemiluminescence.

The Analyst·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Mar 26, 2026

Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis

Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis

Published on: March 7, 2018

Gold nanoparticles as sensitive optical probes.

Zhiqin Yuan¹, Cho-Chun Hu², Huan-Tsung Chang³

¹State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, China. yuanzq@mail.buct.edu.cn.

|February 9, 2016

Summary

This summary is machine-generated.

Gold nanoparticles (Au NPs) offer versatile optical properties for sensitive analyte detection. This review covers their preparation, sensing mechanisms, and signal amplification strategies for analyzing complex samples.

More Related Videos

Synthesis of Near-Infrared Emitting Gold Nanoclusters for Biological Applications

Synthesis of Near-Infrared Emitting Gold Nanoclusters for Biological Applications

Published on: March 22, 2020

Optical Trapping of Nanoparticles

Optical Trapping of Nanoparticles

Published on: January 15, 2013

Related Experiment Videos

Last Updated: Mar 26, 2026

Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis

Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis

Published on: March 7, 2018

Synthesis of Near-Infrared Emitting Gold Nanoclusters for Biological Applications

Synthesis of Near-Infrared Emitting Gold Nanoclusters for Biological Applications

Published on: March 22, 2020

Optical Trapping of Nanoparticles

Optical Trapping of Nanoparticles

Published on: January 15, 2013

Area of Science:

Nanotechnology
Analytical Chemistry
Materials Science

Background:

Gold nanoparticles (Au NPs) are widely used for sensing due to their stability, biocompatibility, and tunable optical properties.
Au NPs enable sensitive and selective detection of various analytes like metal ions, anions, proteins, and DNA.
Analyte detection relies on changes in Au NP absorption, fluorescence, or scattering.

Purpose of the Study:

To provide a tutorial review on gold nanoparticle-based optical sensing systems.
To discuss preparation methods, sensing mechanisms, and signal amplification strategies for Au NP sensors.
To highlight the practical applications and future trends in Au NP optical sensing.

Main Methods:

Brief discussion of wet chemical approaches for Au NP synthesis.
Explanation of sensing mechanisms based on analyte-induced optical property changes.
Emphasis on signal amplification strategies for trace analyte detection.

Main Results:

Demonstration of sensitive and selective optical sensing systems using Au NPs.
Highlighting practical applications in analyzing complex biological and environmental samples.
Review of various Au NP-based optical sensing systems.

Conclusions:

Au NPs are effective tools for developing sensitive and selective optical sensors.
Signal amplification is crucial for detecting trace analytes in real-world samples.
Future trends involve addressing challenges and expanding applications of Au NP optical sensing.