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Updated: Mar 26, 2026

Probing Surface Electrochemical Activity of Nanomaterials using a Hybrid Atomic Force Microscope-Scanning Electrochemical Microscope AFM-SECM

Probing Surface Electrochemical Activity of Nanomaterials using a Hybrid Atomic Force Microscope-Scanning Electrochemical Microscope AFM-SECM

Published on: February 10, 2021

Probing the PEDOT:PSS/cell interface with conductive colloidal probe AFM-SECM.

P Knittel¹, H Zhang², C Kranz¹

¹University of Ulm, Institute of Analytical and Bioanalytical Chemistry, Albert-Einstein-Allee 11, 89081 Ulm, Germany. christine.kranz@uni-ulm.de.

|February 9, 2016

Summary

This summary is machine-generated.

New Atomic Force-Scanning Electrochemical Microscopy (AFM-SECM) probes enable rapid, simultaneous force and electrochemical measurements at the cell-biomaterial interface. These conductive probes allow tunable cell adhesion studies under physiological conditions.

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Functionalization of Atomic Force Microscope Cantilevers with Single-T Cells or Single-Particle for Immunological Single-Cell Force Spectroscopy

Functionalization of Atomic Force Microscope Cantilevers with Single-T Cells or Single-Particle for Immunological Single-Cell Force Spectroscopy

Published on: July 10, 2019

Area of Science:

Biophysics
Materials Science
Electrochemistry

Background:

Atomic Force Microscopy (AFM) is a powerful tool for studying biological systems at the nanoscale.
Existing AFM techniques have limitations in simultaneously measuring mechanical properties and electrochemical signals.
There is a need for advanced AFM probes capable of in-situ electrochemical modification and force measurements.

Purpose of the Study:

To introduce and validate a novel conductive colloidal probe AFM-SECM system.
To demonstrate the ability to perform tunable cell adhesion measurements at the cell-biomaterial interface.
To investigate single-cell interactions as a function of applied electrical potential.

Main Methods:

Development of conductive colloidal probes with a gold-coated colloid at the cantilever end.
Modification of probes via electrochemical polymerization, creating conductive polymer-coated spheres (e.g., PEDOT:PSS).
Simultaneous force measurements (adhesion) and spatially resolved electrochemical information (e.g., oxygen reduction) using AFM-SECM.

Main Results:

Demonstrated successful force measurements under physiological conditions with biased conductive probes.
Showcased rapid adhesion measurements on multiple cells at the cell-biomaterial interface.
Confirmed that polymer properties and cell adhesion can be modulated by applied electrical bias.
Obtained simultaneous spatially resolved electrochemical data.

Conclusions:

Conductive colloid AFM-SECM probes offer a versatile platform for advanced cell-biomaterial interface studies.
The ability to tune probe properties electrochemically opens new avenues for investigating cell mechanics and interactions.
This technique allows for rapid, multi-parameter characterization of single-cell behavior.