Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Satellite Stem Cells and Muscular Dystrophy01:21

Satellite Stem Cells and Muscular Dystrophy

2.5K
Satellite stem cells or myosatellite cells are quiescent stem cells that Alexander Mauro first identified in 1961. These cells are located between the sarcolemma, the plasma membrane of muscle fibers, and the basal lamina, the connective tissue sheath covering it. These mononucleated cells are activated in response to muscle injury, can transform into myoblasts, and may form or repair muscle fibers. Myosatellite cells can provide additional myonuclei for muscle regeneration or return to a...
2.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Retraction notice to 'Sam68 impedes the recovery of arterial injury by augmenting inflammatory response' [Journal of Molecular and Cellular Cardiology 137 (2019) 82-92].

Journal of molecular and cellular cardiology·2026
Same author

Functional characterization of UHRF1 variants in facilitating DNA methylation.

The Journal of biological chemistry·2026
Same author

Divergent Evolutionary Routes of Hypoxia Tolerance in Mangroves.

Molecular ecology·2026
Same author

Acquired resistance to the PRMT5 inhibitor confers collateral sensitivity to MEK inhibition in MTAP-null non-small cell lung cancer.

bioRxiv : the preprint server for biology·2026
Same author

Meeting report on FASEB protein arginine methylation: mechanism to therapeutics.

The Journal of biological chemistry·2026
Same author

Structure of human lymphoid-specific helicase HELLS in its autoinhibited state.

Nucleic acids research·2026

Related Experiment Video

Updated: Mar 26, 2026

Analyzing Satellite Cell Function During Skeletal Muscle Regeneration by Cardiotoxin Injury and Injection of Self-delivering siRNA In Vivo
06:37

Analyzing Satellite Cell Function During Skeletal Muscle Regeneration by Cardiotoxin Injury and Injection of Self-delivering siRNA In Vivo

Published on: September 18, 2019

15.7K

PRMT7 Preserves Satellite Cell Regenerative Capacity.

Roméo Sébastien Blanc1, Gillian Vogel1, Taiping Chen2

  • 1Terry Fox Molecular Oncology Group and Bloomfield Center for Research on Aging, Lady Davis Institute for Medical Research, Sir Mortimer B. Davis Jewish General Hospital, Montréal, QC H3T 1E2, Canada; Departments of Oncology and Medicine, McGill University, Montréal, QC H3T 1E2, Canada.

Cell Reports
|February 9, 2016
PubMed
Summary

Protein arginine methyltransferase 7 (PRMT7) is crucial for muscle stem cell function and regeneration. Loss of PRMT7 impairs satellite cell self-renewal and regeneration by affecting the DNMT3b/p21 axis.

Keywords:
DNMT3bPRMT7agingmuscle regenerationmuscle stem cellp21CIP1senescence

More Related Videos

Isolation of Type I and Type II Pericytes from Mouse Skeletal Muscles
10:07

Isolation of Type I and Type II Pericytes from Mouse Skeletal Muscles

Published on: May 26, 2017

9.6K
Isolation, Culture, and Transplantation of Muscle Satellite Cells
10:25

Isolation, Culture, and Transplantation of Muscle Satellite Cells

Published on: April 8, 2014

43.3K

Related Experiment Videos

Last Updated: Mar 26, 2026

Analyzing Satellite Cell Function During Skeletal Muscle Regeneration by Cardiotoxin Injury and Injection of Self-delivering siRNA In Vivo
06:37

Analyzing Satellite Cell Function During Skeletal Muscle Regeneration by Cardiotoxin Injury and Injection of Self-delivering siRNA In Vivo

Published on: September 18, 2019

15.7K
Isolation of Type I and Type II Pericytes from Mouse Skeletal Muscles
10:07

Isolation of Type I and Type II Pericytes from Mouse Skeletal Muscles

Published on: May 26, 2017

9.6K
Isolation, Culture, and Transplantation of Muscle Satellite Cells
10:25

Isolation, Culture, and Transplantation of Muscle Satellite Cells

Published on: April 8, 2014

43.3K

Area of Science:

  • Muscle biology
  • Stem cell research
  • Epigenetics

Background:

  • Skeletal muscle regeneration depends on quiescent muscle stem cells, known as satellite cells.
  • Satellite cells activate upon injury to repair and regenerate muscle tissue.

Purpose of the Study:

  • To investigate the role of protein arginine methyltransferase 7 (PRMT7) in satellite cell function and muscle regeneration.
  • To elucidate the molecular mechanisms by which PRMT7 influences satellite cell behavior.

Main Methods:

  • Utilized whole-body PRMT7 knockout mice and satellite cell-specific PRMT7 conditional knockout mice (Pax7-CreERT2).
  • Assessed satellite cell function, cell-cycle status, and senescence.
  • Analyzed the expression of key regulatory molecules, including DNMT3b and p21CIP1.

Main Results:

  • PRMT7 deficiency in mice led to reduced satellite cell function and impaired muscle regenerative capacity.
  • PRMT7-deficient satellite cells exhibited cell-cycle arrest and premature senescence.
  • Elevated p21CIP1 and reduced DNMT3b levels were observed in PRMT7-deficient satellite cells.
  • Restoring DNMT3b expression rescued PRMT7-deficient satellite cell senescence.

Conclusions:

  • PRMT7 is essential for maintaining muscle stem cell regenerative capacity.
  • PRMT7 regulates the DNMT3b/p21 axis to prevent satellite cell senescence and ensure proper muscle repair.
  • PRMT7 plays a critical role in the molecular pathways governing muscle stem cell function.