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Conserved SecA Signal Peptide-Binding Site Revealed by Engineered Protein Chimeras and Förster Resonance Energy

Qi Zhang1, Yan Li1, Rich Olson1

  • 1Department of Molecular Biology and Biochemistry, Molecular Biophysics Program, Wesleyan University , Middletown, Connecticut 06459, United States.

Biochemistry
|February 9, 2016
PubMed
Summary

Researchers developed signal peptide-attached SecA chimeras to study bacterial protein transport. This method precisely mapped signal peptide binding sites on the SecA motor protein, revealing crucial interactions for protein translocation.

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