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Design of a Split Intein with Exceptional Protein Splicing Activity

Adam J Stevens1, Zachary Z Brown1, Neel H Shah1

  • 1Department of Chemistry, Princeton University , Frick Laboratory, Princeton, New Jersey 08544, United States.

Journal of the American Chemical Society
|February 9, 2016
PubMed
Summary

Researchers engineered a robust split intein, Cfa, using a consensus design. This novel protein trans-splicing (PTS) system shows enhanced stability and activity, improving biotechnological applications.

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