A review of induced pluripotent stem cell, direct conversion by trans-differentiation, direct reprogramming and
Ankshita Prasad1, Janani Manivannan2, Daniel T B Loong1
1Departments of Orthopaedic Surgery, Biomedical Engineering & Medicine, Division of Neurology, Singapore Institute of Neurotechnology, National University of Singapore, Singapore.
Abstract:
Rapid progress in the field of stem cell therapy and cellular reprogramming provides convincing evidence of its feasibility in treating a wide range of pathologies through autologous cell replacement therapy. This review article describes in detail on three widely used approaches of somatic cell reprogramming: induced pluripotent stem cells, direct conversion and direct reprogramming, in the context of demyelination in the CNS. The potential limitations of each reprogramming technique are reviewed along with their distinct molecular approach to reprogramming. This is followed by an analysis on the scopes and challenges of its translational applications in deriving oligodendrocyte progenitor cells and oligodendrocytes for cell replacement treatment of demyelinating conditions in the CNS.
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