Related Experiment Video

Updated: Mar 26, 2026

Primary Culture of Mouse Dopaminergic Neurons
11:58

Primary Culture of Mouse Dopaminergic Neurons

Published on: September 8, 2014

39.8K

Neuroprotection by Polynitrogen Manganese Complexes: Regulation of Reactive Oxygen Species-Related Pathways

Chunxia Chen1, Jing Cao1, Xiaoyan Ma1

  • 1State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Collaborative Innovation Center of Advanced Microstructures, Nanjing University, Nanjing 210093, P.R. China.

Scientific Reports
|February 10, 2016
PubMed

Insights

Two novel manganese complexes offer neuroprotection against oxidative stress by regulating reactive oxygen species (ROS) levels. These compounds show promise for treating neurologic diseases by modulating cellular responses to H2O2-induced apoptosis.

Area of Science:

  • Neuroscience
  • Biochemistry
  • Pharmacology

Background:

  • Cell death in the central nervous system is implicated in neurologic diseases.
  • Reactive oxygen species (ROS) critically influence cellular oxidative stress and tolerance.
  • Regulating ROS levels presents a therapeutic strategy for neurologic disorders.

Purpose of the Study:

  • To investigate the neuroprotective effects of two polynitrogen manganese complexes (1 and 2) against H2O2-induced apoptosis in PC12 cells and neurons.
  • To explore the ROS-related mechanisms underlying the neuroprotective actions of these complexes.
  • To evaluate the potential of these complexes in modulating intracellular ROS levels for therapeutic benefit.

Main Methods:

  • Preconditioning with polynitrogen manganese complexes (1 and 2).
  • Induction of apoptosis using hydrogen peroxide (H2O2).
  • Assessment of cell viability and apoptosis.
  • Analysis of intracellular ROS levels and antioxidase activity.
  • Investigation of hypoxia-inducible factor (HIF)-related pathways.

Main Results:

  • Both complexes attenuated H2O2-induced apoptosis in PC12 cells and neurons.
  • Complex 1 eliminated intracellular ROS and activated the antioxidase system.
  • Complex 2 generated low levels of ROS, promoting cell survival.
  • Both complexes activated hypoxia-inducible factor-related pathways, enhancing cellular adaptation to oxidative stress.

Conclusions:

  • Polynitrogen manganese complexes demonstrate significant neuroprotective potential.
  • Complexes modulate intracellular ROS levels through distinct mechanisms, offering therapeutic avenues.
  • Targeting ROS pathways with these complexes represents a promising strategy for treating oxidative stress-related neurologic diseases.

More Related Videos

In vivo Imaging of Optic Nerve Fiber Integrity by Contrast-Enhanced MRI in Mice11:38

In vivo Imaging of Optic Nerve Fiber Integrity by Contrast-Enhanced MRI in Mice

Published on: July 22, 2014

14.0K
Setup of Capillary Electrophoresis-Inductively Coupled Plasma Mass Spectrometry CE-ICP-MS for Quantification of Iron Redox Species FeII, FeIII04:48

Setup of Capillary Electrophoresis-Inductively Coupled Plasma Mass Spectrometry CE-ICP-MS for Quantification of Iron Redox Species FeII, FeIII

Published on: May 4, 2020

8.8K

Related Concept Videos

Electron Transport Chain: Complex I and II01:46

Electron Transport Chain: Complex I and II

The mitochondrial electron transport chain (ETC) is the main energy generation system in the eukaryotic cells. However, mitochondria also produce cytotoxic reactive oxygen species (ROS) due to the large electron flow during oxidative phosphorylation. While Complex I is one of the primary sources of superoxide radicals, ROS production by Complex II is uncommon and may only be observed in cancer cells with mutated complexes.
ROS generation is regulated and maintained at moderate levels necessary...
19.5K
Microbes and Other Elemental Cycles01:24

Microbes and Other Elemental Cycles

Microbial activity plays a pivotal role in the biogeochemical cycling of iron and manganese, especially at the redox gradients characteristic of stratified aquatic environments. These cycles are driven by microbial transformations between oxidized and reduced forms of the metals, allowing organisms to exploit them for metabolic energy and structural purposes.Iron Cycling Across Redox GradientsIn neutral, oxygen-rich surface waters, iron is predominantly found in its oxidized, insoluble ferric...
42
The Electron Transport Chain01:30

The Electron Transport Chain

The electron transport chain or oxidative phosphorylation is an exothermic process in which free energy released during electron transfer reactions is coupled to ATP synthesis. This process is a significant source of energy in aerobic cells, and therefore inhibitors of the electron transport chain can be detrimental to the cell's metabolic processes.
Inhibitors of the electron transport chain
Rotenone, a widely used pesticide, prevents electron transfer from Fe-S cluster to ubiquinone or Q...
21.3K
Neurogenesis and Regeneration of Nervous Tissue01:15

Neurogenesis and Regeneration of Nervous Tissue

In the CNS, neurogenesis, the birth of new neurons from stem cells, is limited to the hippocampus in adults. In other regions of the brain and spinal cord, neurogenesis is almost non-existent due to inhibitory influences from neuroglia, especially oligodendrocytes, and the absence of growth-stimulating cues. The myelin produced by oligodendrocytes in the CNS inhibits neuronal regeneration. Furthermore, astrocytes proliferate rapidly after neuronal damage, forming scar tissue that physically...
2.0K