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RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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Ribosome Profiling02:24

Ribosome Profiling

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
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Related Experiment Video

Updated: Mar 26, 2026

Methods to Investigate the Regulatory Role of Small RNAs and Ribosomal Occupancy of Plasmodium falciparum
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Methods to Investigate the Regulatory Role of Small RNAs and Ribosomal Occupancy of Plasmodium falciparum

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New insights into the Plasmodium vivax transcriptome using RNA-Seq.

Lei Zhu1, Sachel Mok1, Mallika Imwong2,3

  • 1School of Biological Sciences, Nanyang Technological University, Singapore.

Scientific Reports
|February 10, 2016
PubMed
Summary

Plasmodium vivax malaria causes significant illness, but its biology is poorly understood. This study maps the Plasmodium vivax transcriptome during its intraerythrocytic developmental cycle, revealing insights into gene regulation and immune evasion.

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Area of Science:

  • Genomics
  • Parasitology
  • Molecular Biology

Background:

  • Plasmodium vivax malaria is increasingly recognized for causing substantial morbidity.
  • Limited understanding of P. vivax biology hinders effective control strategies.
  • The intraerythrocytic developmental cycle (IDC) is crucial for parasite propagation.

Purpose of the Study:

  • To establish a high-resolution transcriptome map of the P. vivax IDC.
  • To gain insights into gene regulatory mechanisms and biological functions.
  • To identify potential targets for immune evasion and transcriptional regulation.

Main Methods:

  • Illumina HiSeq platform for high-resolution transcriptome sequencing.
  • Analysis of two clinical isolates of P. vivax.
  • Comparative genomic analysis with other eukaryotes.

Main Results:

  • Detailed transcriptome map of P. vivax IDC.
  • Identification of a transcriptional hotspot of vir genes on chromosome 2, potentially involved in immune evasion.
  • P. vivax genes exhibit unusually long 5' UTRs and multiple transcription start sites.
  • Rare alternative splicing associated with the late schizont stage.
  • Discovery of 179 vir-like genes and 3018 noncoding RNAs.

Conclusions:

  • The P. vivax transcriptome map provides novel insights into parasite biology.
  • Vir genes and noncoding RNAs may play significant roles in strain-specific transcriptional regulation and immune evasion.
  • Understanding these elements is crucial for developing targeted malaria control strategies.