Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
Uniform Depth Channel Flow01:27

Uniform Depth Channel Flow

Uniform depth channel flow keeps fluid depth consistent along channels such as irrigation canals. In natural channels, such as rivers, approximate uniform flow is often assumed. This condition occurs when the channel’s bottom slope matches the energy slope, balancing potential energy lost from gravity with head loss due to shear stress. This balance prevents depth changes along the channel length, resulting in a steady, uniform flow.Uniform flow in open channels with a constant cross-section...
Uniform Depth Channel Flow: Problem Solving01:18

Uniform Depth Channel Flow: Problem Solving

To calculate the flow rate for a trapezoidal channel, first, identify the bottom width, side slope, and flow depth of the channel. The cross-sectional area (A) corresponding to the depth of flow (y), channel bottom width (B), and side slope (θ) is determined by:Next, calculate the wetted perimeter, which includes the bottom width and the sloped side lengths in contact with the water. Using the values of the cross-sectional area and the wetted perimeter, determine the hydraulic radius by...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Development and Fabrication of Biocompatible Ti-Based Bulk Metallic Glass Matrix Composites for Additive Manufacturing.

Materials (Basel, Switzerland)·2023
Same author

Microfluidic chip for droplet-based AuNP synthesis with dielectric barrier discharge plasma and on-chip mercury ion detection.

RSC advances·2022
Same author

Observing the three-dimensional terephthalic acid supramolecular growth mechanism on a stearic acid buffer layer by molecular simulation methods.

RSC advances·2022
Same author

Design of Customize Interbody Fusion Cages of Ti64ELI with Gradient Porosity by Selective Laser Melting Process.

Micromachines·2021
Same author

Multiple enzyme-doped thread-based microfluidic system for blood urea nitrogen and glucose detection in human whole blood.

Biomicrofluidics·2015

Related Experiment Video

Updated: Jun 23, 2026

Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects
10:16

Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects

Published on: February 8, 2014

12.7K

Depth position detection for fast moving objects in sealed microchannel utilizing chromatic aberration.

Che-Hsin Lin1, Shin-Yu Su1

  • 1Department of Mechanical and Electro-Mechanical Engineering, National Sun Yat-sen University , Kaohsiung 804, Taiwan.

Biomicrofluidics
|February 10, 2016
PubMed
Summary
This summary is machine-generated.

This study introduces a new, low-cost method for measuring the depth of fast-moving objects in microfluidic channels using chromatic aberration. The technique successfully resolves small particles and detects human red blood cells at high speeds without labeling.

More Related Videos

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells
11:06

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Published on: June 30, 2018

9.2K
Conducting Multiple Imaging Modes with One Fluorescence Microscope
08:32

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

10.4K

Related Experiment Videos

Last Updated: Jun 23, 2026

Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects
10:16

Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects

Published on: February 8, 2014

12.7K
Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells
11:06

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Published on: June 30, 2018

9.2K
Conducting Multiple Imaging Modes with One Fluorescence Microscope
08:32

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

10.4K

Area of Science:

  • Optics and Photonics
  • Microfluidics
  • Biomedical Engineering

Background:

  • Accurate depth position measurement of fast-moving objects in microfluidic channels is challenging.
  • Existing methods may lack speed, resolution, or simplicity for real-time applications.

Purpose of the Study:

  • To develop a novel, cost-effective method for depth position measurement of rapid objects within microfluidic systems.
  • To leverage the chromatic aberration effect for quantitative depth analysis.

Main Methods:

  • Utilized chromatic aberration by employing lenses with different Abbe numbers (PMMA and BK7).
  • Employed two band-pass filters (blue and red) and two avalanche photodiodes (APDs) to detect scattered light.
  • Analyzed the intensity ratio of blue and red light bands to determine depth information.

Main Results:

  • Successfully resolved 20 μm microspheres with PMMA and 2 μm microspheres with BK7.
  • Achieved high throughput detection due to sensitive APDs.
  • Detected human erythrocytes at 2.8 mm/s without fluorescence labeling.

Conclusions:

  • The developed method provides a simple, low-cost solution for quantitative depth measurement of fast-moving objects in microfluidic channels.
  • The system demonstrates high sensitivity and throughput, suitable for various applications including cell analysis.
  • Chromatic aberration offers a viable principle for optical sensing in microfluidic devices.