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Related Concept Videos

Laminins are the Adhesive Proteins of Basal Lamina00:55

Laminins are the Adhesive Proteins of Basal Lamina

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Laminins are heterotrimeric proteins with high molecular mass found in the extracellular matrix. Each laminin molecule is composed of three chains, viz. alpha, beta, and gamma, coded by five, four, and three paralogous genes, respectively. Laminins are categories based on the compositions of the three chains.
In humans, the five forms of alpha chains are LAMA 1, LAMA 2, LAMA 3, LAMA 4, and LAMA 5. The four forms of beta chains are LAMB 1, LAMB 2, LAMB 3, and LAMB 4. The three forms of gamma...
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The endoplasmic reticulum (ER) of pancreatic β-cells synthesizes preproinsulin, which consists of a signal peptide, A and B chains, and a C-peptide. Preproinsulin is then cleaved and folded into proinsulin, which translocates to the Golgi apparatus for sorting and packaging into secretory granules. In these granules, enzymatic clipping generates insulin and C-peptide.
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Related Experiment Video

Updated: Mar 26, 2026

Generating a Fractal Microstructure of Laminin-111 to Signal to Cells
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Human Laminin Isotype Coating for Creating Islet Cell Sheets.

Shingo Yamashita1, Kazuo Ohashi2, Rie Utoh3

  • 1Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, Shinjuku-ku, Tokyo, Japan; †Department of Surgery, Institute of Gastroenterology, Tokyo Women's Medical University, Shinjuku-ku, Tokyo, Japan.

Cell Medicine
|February 10, 2016
PubMed
Summary

Researchers developed an optimal human extracellular matrix (ECM), human laminin-332 (HL-332), for coating temperature-responsive polymers. This innovation enables the creation of functional islet cell sheets for potential diabetes mellitus treatments.

Keywords:
Cell sheetDispersed islet cellsExtracellular matrix (ECM)LamininPancreatic isletsTemperature-responsive polymer

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Area of Science:

  • Biomaterials Science
  • Regenerative Medicine
  • Endocrinology

Background:

  • Developing islet cell sheets for diabetes treatment requires suitable extracellular matrix (ECM) coatings.
  • Rat laminin-5 on poly(N-isopropylacrylamide) (PIPAAm) has shown promise, but human-derived materials are needed for clinical application.

Purpose of the Study:

  • To identify an optimal human ECM for coating PIPAAm surfaces to facilitate islet cell attachment and harvesting as a monolithic sheet.
  • To evaluate different human laminin isotypes for their efficacy in islet cell culture.

Main Methods:

  • Rat islet cells were cultured on PIPAAm dishes coated with various human laminin isotypes (HL-211, HL-332, HL-411, HL-511, HL-placenta).
  • Assessed plating efficiency, cellular confluency, and glucose-stimulated insulin secretion.
  • Evaluated the ability to harvest cells as a monolithic sheet by temperature reduction.

Main Results:

  • Human laminin-332 (HL-332) coated PIPAAm demonstrated the highest plating efficiency (83.1%) and cellular confluency (98.6%).
  • Islet cells cultured on HL-332-PIPAAm showed positive glucose-stimulated insulin secretion.
  • Monolithic islet cell sheets were successfully harvested from HL-332-PIPAAm surfaces at 20°C.

Conclusions:

  • Human laminin-332 (HL-332) is an optimal human-derived ECM for PIPAAm coatings in preparing islet cell sheets.
  • This HL-332-PIPAAm system supports islet cell function and enables sheet harvesting for potential diabetes therapies.