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DNAzyme-dependent Analysis of rRNA 2’-O-Methylation
Published on: September 16, 2019
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Isotope effect analyses provide evidence for an altered transition state for RNA 2'-O-transphosphorylation catalyzed
Shuming Zhang1, Hong Gu1, Haoyuan Chen2
1Department of Biochemistry, Case Western Reserve University School of Medicine, Cleveland, OH 44106, USA. meh2@cwru.edu.
Chemical Communications (Cambridge, England)
|February 10, 2016
Abstract:
Solvent D2O and (18)O kinetic isotope effects on RNA 2'-O-transphosphorylation catalyzed by Zn(2+) demonstrate an altered transition state relative to specific base catalysis. A recent model from DFT calculations involving inner sphere coordination to the non-bridging and leaving group oxygens is consistent with the data.
