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Phage Therapy Application to Counteract Pseudomonas aeruginosa Infection in Cystic Fibrosis Zebrafish Embryos
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[New Virulent Bacteriophages Active against Multiresistant Pseudomonas aeruginosa Strains]

N Sh Balarjishvili, L I Kvachadze, M I Kutateladze

    Prikladnaia Biokhimiia I Mikrobiologiia
    |February 11, 2016
    PubMed

    Insights

    New bacteriophages effectively target antibiotic-resistant Pseudomonas aeruginosa. These phages show high efficacy against multi-drug resistant strains, suggesting a promising phage therapy approach.

    Area of Science:

    • Microbiology
    • Virology
    • Genetics

    Background:

    • Pseudomonas aeruginosa is a significant opportunistic pathogen, frequently exhibiting antibiotic resistance.
    • The rise of multi-drug resistant (MDR) strains necessitates novel therapeutic strategies.

    Purpose of the Study:

    • To isolate and characterize novel bacteriophages targeting antibiotic-resistant Pseudomonas aeruginosa.
    • To evaluate the efficacy of these bacteriophages as a potential therapeutic agent against MDR P. aeruginosa.

    Main Methods:

    • Isolation and characterization of three novel virulent bacteriophages (Myoviridae, Podoviridae) against P. aeruginosa.
    • Genotyping of antibiotic-resistant strains and analysis of phage-host interactions.
    • Determination of phage genome molecular weight, DNA restriction analysis, and protein electrophoresis.
    • Assessment of phage viability under various inactivating factors (temperature, pH, UV).
    • Evaluation of phage lytic activity against a panel of antibiotic-resistant and MDR P. aeruginosa strains.

    Main Results:

    • Three novel virulent bacteriophages were isolated from P. aeruginosa clinical strains.
    • Phage development cycle parameters and viability under stress conditions were determined.
    • Phages demonstrated high lytic efficacy against 26 out of 28 genetically distinct antibiotic-resistant P. aeruginosa strains.
    • A multicomponent phage preparation showed enhanced antibacterial activity against 427 MDR clinical isolates.

    Conclusions:

    • The isolated bacteriophages are effective against a broad spectrum of antibiotic-resistant P. aeruginosa.
    • Phage therapy, particularly using multicomponent preparations, presents a viable strategy to combat MDR P. aeruginosa infections.

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