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Isolating, Sequencing and Analyzing Extracellular MicroRNAs from Human Mesenchymal Stem Cells
Published on: March 8, 2019
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What is normal? Next generation sequencing-driven analysis of the human circulating miRNAOme
1School of Life Sciences, Faculty of Natural Sciences, Keele University, Keele, ST5 5BG, UK. d.p.tonge@keele.ac.uk.
BMC Molecular Biology
|February 11, 2016
Summary
This study characterized circulating microRNAs (miRNAs) in healthy individuals using sequencing, identifying key miRNA species and their expression levels. These findings provide essential baseline data for miRNA biomarker discovery in plasma.
Area of Science:
- Molecular Biology
- Genomics
- Biochemistry
Background:
- MicroRNAs (miRNAs) are key regulators of gene expression, impacting numerous physiological processes.
- miRNAs are detectable and stable in biological fluids, offering potential as biomarkers.
- Limited baseline data hinders the validation of miRNA biomarkers for disease diagnosis and therapy response.
Purpose of the Study:
- To determine the global expression profile of circulating miRNAs in human plasma.
- To establish baseline data for miRNA biomarker discovery and validation.
Main Methods:
- Utilized state-of-the-art sequencing technologies.
- Analyzed plasma samples from 18 disease-free human subjects.
- Quantified global miRNA expression and identified stable miRNA species.
Main Results:
- Detected over 500 unique miRNAs in plasma.
- Ten highly expressed miRNAs constituted 90% of total miRNA reads.
- Determined expression ranges for all detected miRNAs and identified highly stable ones.
- Investigated the influence of gender, smoking, and BMI on miRNA expression.
Conclusions:
- Provides crucial baseline expression data for circulating miRNAs in plasma.
- Facilitates the identification and validation of miRNA biomarkers for disease screening.
- Aims to expedite miRNA biomarker discovery and reduce associated research costs.
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