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Updated: Mar 26, 2026

Measurement of Tissue Non-Heme Iron Content using a Bathophenanthroline-Based Colorimetric Assay
Published on: January 31, 2022
Visual Assay of Total Iron in Human Serum with Bathophenanthrolin Disulfonate-accommodated MCM-41
Misato Sakamoto1, Keita Hizawa, Manabu Hosaka
1Department of Chemistry, College of Humanities and Sciences, Nihon University.
Abstract:
A simple visual method for determining the total iron in human serum is proposed based on color development in the nanospace of mesoporous silica MCM-41 and a chromogenic ligand bathophenathroline disulfonate (BPS). Observing the color intensity of a complex between iron(II) and BPS devloped on the MCM-41 material by the naked eye enabled us to quntify iron(II) with a detection limit of 0.5 μM. The BPS-loaded MCM-41 was successfully applied for quantifying the total iron in human serum.

