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Dose Dependent Effect of Iso-Octane on HaCaT: A Model Study
Lopamudra Das1, Soumen Das1, Jyotirmoy Chatterjee1
1School of Medical Science and Technology, Indian Institute of Technology, Kharagpur, West Bengal, India.
Toxicology International
|February 11, 2016
Summary
Iso-octane (IO) exposure causes dose-dependent cytotoxicity in HaCaT cells, decreasing viability and altering cellular structure. This study highlights a multimodal approach for evaluating epithelial toxicity in vitro.
Area of Science:
- Toxicology
- Cell Biology
- Biophysics
Background:
- Understanding chemical assaults on cells is crucial for safety assessments.
- Multimodal analysis offers a comprehensive approach to evaluating cytotoxicity.
Purpose of the Study:
- To investigate the dose-dependent cytotoxicity of iso-octane (IO) on HaCaT cells.
- To explore a multimodal analytical approach for assessing epithelial toxicity in vitro.
Main Methods:
- HaCaT cells were exposed to varying doses of iso-octane (IO).
- Cell viability, morphology, E-cadherin expression, bioelectrical properties, and biomechanical properties were analyzed.
- Immunocytochemistry, transcriptomics, and micropipette aspiration were employed.
Main Results:
- Iso-octane (IO) exposure led to a dose-dependent decrease in HaCaT cell viability.
- E-cadherin expression decreased, and it translocated from the membrane to the cytoplasm.
- Significant changes in bioelectrical and biomechanical properties, including increased cellular stiffness, were observed.
Conclusions:
- Iso-octane (IO) exhibits dose-dependent cytotoxicity on HaCaT cells.
- A multimodal approach can effectively evaluate epithelial toxicity in vitro.
- This method provides level-free markers for assessing toxicity.
