Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Reproductive Cloning01:27

Reproductive Cloning

33.2K
Reproductive cloning is the process of producing a genetically identical copy—a clone—of an entire organism. While clones can be produced by splitting an early embryo—similar to what happens naturally with identical twins—cloning of adult animals is usually done by a process called somatic cell nuclear transfer (SCNT).
Somatic Cell Nuclear Transfer
In SCNT, an egg cell is taken from an animal and its nucleus is removed, creating an enucleated egg. Then a somatic...
33.2K
Introduction to Nuclear Reprogramming01:14

Introduction to Nuclear Reprogramming

2.4K
Nuclear reprogramming is the process of switching gene expression of one cell type to that of another cell type, usually from a differentiated cell state to an undifferentiated cell state. Differentiation occurs during processes such as development and morphogenesis, tissue regeneration, and malignancy. Cells can also be artificially induced to reprogram their gene expression by techniques such as nuclear transfer, induced pluripotency, and cell fusion. Such techniques have many applications in...
2.4K
Cloning of Dolly the Sheep01:08

Cloning of Dolly the Sheep

8.9K
The first successfully cloned mammal was Dolly, a sheep, born on 5th July 1996 at Roslin Institute, Scotland. The cloned sheep was named after the American singer Dolly Parton. Dolly lived for seven years and died of respiratory complications, which is speculated to be due to the actual age of her DNA. Because the DNA in cloned cells belongs to an older individual,  the cloned individual’s life expectancy may be affected. Indeed, analysis of Dolly’s DNA revealed shorter...
8.9K
Methods of Nuclear Reprogramming01:24

Methods of Nuclear Reprogramming

2.2K
Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for...
2.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Modelling human genetic disorders in Xenopus tropicalis.

Disease models & mechanisms·2024
Same author

Erratum: Best Practices for <i>Xenopus tropicalis</i> Husbandry.

Cold Spring Harbor protocols·2023
Same author

Report on the 2021 Aniridia North America symposium on PAX6, aniridia, and beyond.

The ocular surface·2023
Same author

Production of Transgenic F<sub>0</sub> Animals and Permanent Lines by Sperm Nuclear Transplantation in <i>Xenopus tropicalis</i>.

Cold Spring Harbor protocols·2022
Same author

Best Practices for <i>Xenopus tropicalis</i> Husbandry.

Cold Spring Harbor protocols·2022
Same author

Homology-Directed Repair by CRISPR-Cas9 Mutagenesis in <i>Xenopus</i> Using Long Single-Stranded Donor DNA Templates via Simple Microinjection of Embryos.

Cold Spring Harbor protocols·2022
Same journal

A Video Protocol of a Randomized Controlled Clinical Trial - Electrochemotherapy of Cutaneous Metastases with Reduced Dose Bleomycin (BLESS Trial).

Journal of visualized experiments : JoVE·2026
Same journal

A Standardized Ex Vivo Porcine Oromucosal Model for Evaluating Peptide Fluxes.

Journal of visualized experiments : JoVE·2026
Same journal

Lightweight English Text Classification with Deep Learning Based on Complex System Theory.

Journal of visualized experiments : JoVE·2026
Same journal

Integrating Artificial Intelligence-Assisted Translation Support into English Courses: Effects on Translation Accuracy, Perceived Stress, and Anxiety.

Journal of visualized experiments : JoVE·2026
Same journal

A Toxin-Based Counter-Selection System for Markerless Gene Deletion and High-Density Tn5 Transposon Mutagenesis in Pectobacterium brasiliense.

Journal of visualized experiments : JoVE·2026
Same journal

Seamless Multimodal Human-Robot Communication: Integration Techniques in Human-Computer Interaction.

Journal of visualized experiments : JoVE·2026
See all related articles

Related Experiment Video

Updated: Mar 26, 2026

Functional Cloning Using a Xenopus Oocyte Expression System
09:40

Functional Cloning Using a Xenopus Oocyte Expression System

Published on: January 30, 2016

8.7K

Functional Cloning Using a Xenopus Oocyte Expression System.

Carol Zygar Plautz1, Hannah C Williams2, Robert M Grainger3

  • 1Department of Biology, Shepherd University; CPLAUTZ@shepherd.edu.

Journal of Visualized Experiments : Jove
|February 11, 2016
PubMed
Summary
This summary is machine-generated.

Researchers identified ldb1 as a key gene in embryonic lens induction using Xenopus oocytes. This functional cloning approach helps discover novel signaling molecules and regulatory proteins involved in early development.

More Related Videos

Xenopus Oocytes: Optimized Methods for Microinjection, Removal of Follicular Cell Layers, and Fast Solution Changes in Electrophysiological Experiments
07:24

Xenopus Oocytes: Optimized Methods for Microinjection, Removal of Follicular Cell Layers, and Fast Solution Changes in Electrophysiological Experiments

Published on: December 31, 2016

21.1K
Production of Transgenic Xenopus laevis by Restriction Enzyme Mediated Integration and Nuclear Transplantation
09:48

Production of Transgenic Xenopus laevis by Restriction Enzyme Mediated Integration and Nuclear Transplantation

Published on: August 21, 2010

12.5K

Related Experiment Videos

Last Updated: Mar 26, 2026

Functional Cloning Using a Xenopus Oocyte Expression System
09:40

Functional Cloning Using a Xenopus Oocyte Expression System

Published on: January 30, 2016

8.7K
Xenopus Oocytes: Optimized Methods for Microinjection, Removal of Follicular Cell Layers, and Fast Solution Changes in Electrophysiological Experiments
07:24

Xenopus Oocytes: Optimized Methods for Microinjection, Removal of Follicular Cell Layers, and Fast Solution Changes in Electrophysiological Experiments

Published on: December 31, 2016

21.1K
Production of Transgenic Xenopus laevis by Restriction Enzyme Mediated Integration and Nuclear Transplantation
09:48

Production of Transgenic Xenopus laevis by Restriction Enzyme Mediated Integration and Nuclear Transplantation

Published on: August 21, 2010

12.5K

Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Genetics

Background:

  • Identifying embryonic inducers is challenging due to low abundance, cell-tethering, or requirement for binding partners.
  • Existing methods may not efficiently identify paracrine, juxtacrine, or regulatory factors.

Purpose of the Study:

  • To develop and validate a functional cloning system for identifying gene products that elicit embryonic inductive responses.
  • To discover novel factors involved in early lens induction in Xenopus.

Main Methods:

  • Utilized an expression cloning system involving Xenopus oocytes and co-cultured responding tissue.
  • Injected pools of mRNA into oocytes and screened for specific developmental responses.
  • Functionally cloned ldb1 based on its ability to induce foxe3 expression in competent ectoderm.

Main Results:

  • Successfully identified ldb1 using the functional cloning system.
  • Demonstrated ldb1's role in inducing the early lens placode marker foxe3.
  • Characterized ldb1 mRNA expression and developmental effects of its overexpression and knockdown.

Conclusions:

  • The developed Xenopus oocyte-based expression cloning system is effective for identifying embryonic inducers and regulatory molecules.
  • ldb1 plays a significant role in early lens induction.
  • This system has broad applicability for discovering novel factors in various developmental contexts.