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No Evidence That RFamide-Related Peptide 3 Directly Modulates LH Secretion in the Ewe.

C Decourt¹, K Anger¹, V Robert¹

¹Unité Mixte de Recherche Physiologie de la Reproduction et des Comportements (Institut National de la Recherche Agronomique, Unité Mixte de Recherche 85; Centre National de la Recherche Scientifique, Unité Mixte de Recherche 7247, Université François Rabelais Tours, IFCE) (C.D., K.A., V.R., D.L., J.B.-S., A.C., L.D., M.B.), F-37380 Nouzilly, France; and Centre for Neuroendocrinology and Department of Anatomy (G.A.), University of Otago School of Medical Sciences, Dunedin 9054, New Zealand.

|February 11, 2016

Summary

Neuropeptide RFamide-related peptide 3 (RFRP-3) likely has a minor role in controlling LH secretion in ewes. Studies found RFRP-3 did not directly affect LH levels, and its antagonist

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Area of Science:

Reproductive Endocrinology
Neuroendocrinology
Mammalian Physiology

Background:

Neuropeptide RFamide-related peptide 3 (RFRP-3) is known to influence gonadotropin secretion in various species.
Previous studies in ewes reported conflicting effects of RFRP-3 on luteinizing hormone (LH) levels, ranging from inhibitory to no effect.
The role of RFRP-3 in LH secretion requires clarification due to inconsistent findings and the impact of antagonists like RF9.

Purpose of the Study:

To investigate the direct effect of RFRP-3 on LH secretion in ewes.
To clarify the conflicting results regarding RFRP-3's role in reproductive hormone regulation.
To determine if RF9's effect on LH release is mediated through the RFRP-3 receptor.

Main Methods:

Administered RFRP-3 via intravenous injections and continuous perfusion in ovariectomized and intact ewes.
Evaluated LH pulsatility and surge responses following RFRP-3 administration.
Compared the effects of RF9 and a selective RFRP-3 receptor antagonist (GJ14) on LH release.

Main Results:

Multiple RFRP-3 injections and perfusions did not alter plasma LH pulsatility or LH surges in ewes.
RFRP-3 did not affect the LH pulse induced by kisspeptin or the LH surge induced by estradiol benzoate.
While RF9 increased LH release, the selective antagonist GJ14 showed no significant effect on LH.

Conclusions:

RFRP-3 appears to have no direct effect on LH secretion in ewes.
The observed effects of RF9 on LH release are likely not mediated by the RFRP-3 receptor.
RFRP-3 probably plays a minor role in the control of LH secretion in ewes.