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Updated: Mar 26, 2026

Metabolic Pathway Confirmation and Discovery Through 13C-labeling of Proteinogenic Amino Acids
Published on: January 26, 2012
C3 and 2D C3 Marfey's Methods for Amino Acid Analysis in Natural Products
Soumini Vijayasarathy1, Pritesh Prasad1, Leith J Fremlin1
1Institute for Molecular Bioscience, The University of Queensland , St. Lucia, Queensland 4072, Australia.
Abstract:
We validate the improved resolution and sensitivity of the C3 Marfey's method, including an ability to resolve all Ile isomers, against an array of amino acids commonly encountered in natural products and by comparison to an existing Marfey's method. We also describe an innovative 2D C3 Marfey's method as an analytical approach for determining the regiochemistry of enantiomeric amino acid residues in natural products. The C3 and 2D C3 Marfey's methods represent valuable tools for probing and defining the stereocomplexity of hydrolytically accessible amino acid residues in natural products.
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