Related Experiment Video
Updated: Mar 26, 2026
Chronic Salmonella Infection Induced Intestinal Fibrosis
Published on: September 22, 2019
Disease duration and age influence CARD15 expression in Crohn's disease
Elżbieta Poniewierka1, Katarzyna Neubauer2, Radosław Kempiński2
1Department of Gastroenterology and Hepatology, Wroclaw Medical University, Poland, 50-556 Wroclaw, Borowska 213; Department of Gastroenterology and Hepatology. Dietetics Unit, Wroclaw Medical University.
Insights
CARD15 gene expression is elevated in Crohn's disease (CD) patients compared to healthy individuals. Expression levels correlate with disease duration and patient age, decreasing with anti-TNFα therapy.
Area of Science:
- Immunogenetics
- Gastroenterology
Background:
- CARD15 is a known susceptibility gene for Crohn's disease (CD).
- Understanding CARD15 expression patterns in CD is crucial for disease management.
Purpose of the Study:
- To investigate the relationship between peripheral CARD15 gene expression and various CD phenotypes.
- To analyze the influence of disease duration, age, treatment, and inflammatory markers on CARD15 expression.
Main Methods:
- Quantitative real-time PCR was used to measure CARD15 mRNA levels in peripheral blood mononuclear cells (PBMCs).
- Sixty CD patients and 30 healthy controls were included.
- Correlations with clinical data including disease duration, age, treatment (anti-TNFα, azathioprine, steroids), ESR, and CRP were assessed.
Main Results:
- CARD15 gene expression was significantly higher in CD patients than in controls.
- Expression peaked in CD patients in their fourth decade of life and was elevated in those with 12-60 months disease duration.
- A positive correlation was observed between CARD15 expression and erythrocyte sedimentation rate (ESR).
- Gene expression decreased significantly in patients treated with anti-TNFα agents compared to azathioprine or steroid treatments.
Conclusions:
- Peripheral CARD15 expression is elevated in Crohn's disease patients.
- Disease duration and patient age are key factors influencing CARD15 expression levels.
- Anti-TNFα therapy is associated with reduced CARD15 expression in CD patients.
Abstract:
One of the susceptibility genes in Crohn's disease (CD) is CARD15. Our study examined the relationship between peripheral CARD15 expression and phenotype and duration of CD, treatment methods and inflammatory indices. Sixty patients with CD and 30 healthy volunteers as controls were enrolled in the study. Total RNA was isolated from peripheral blood mononuclear cells (PBMCs) with E.Z.N.A. Total RNA Kit (Omega Bio-tek) then quantitative real-time PCR was performed on the ABI Prism 7900 HT Real-Time PCR System. CARD15 gene expression in PBMCs in CD was significantly higher than in the control group. The highest level of gene expression was found in CD patients in the fourth decade of life. The mRNA level of the CARD15 gene was higher in patients with disease duration between 12 and 60 months. A positive correlation was found between erythrocyte sedimentation rate (ESR) and gene expression level. Gene expression increased with increasing level of C-reactive protein and ESR, but it was not statistically significant. CARD15 expression significantly decreased in CD patients treated with anti-TNFα agents compared to azathioprine or steroid treatment groups. Expression of the CARD15 gene in Crohn›s disease is higher than in healthy individuals. Disease duration and age of patients seem to be the most important factors influencing CARD15 expression.
More Related Videos
05:08A TNBS-Induced Rodent Model to Study the Pathogenic Role of Mechanical Stress in Crohn's Disease
Published on: March 1, 2022
10:21Mechanistic Insight into the Development of TNBS-Mediated Intestinal Fibrosis and Evaluating the Inhibitory Effects of Rapamycin
Published on: September 12, 2019
Related Concept Videos
01:30Inflammatory Bowel Disease II: Crohn's Disease
Inflammatory bowel disease, commonly known as IBD, refers to a collection of disorders that lead to persistent inflammation of the gastrointestinal tract. The two types of IBD are ulcerative colitis, which impacts the colon, and Crohn's disease, which can involve any part of the gastrointestinal segment.
Crohn's disease
Crohn's disease is a chronic, systemic inflammatory bowel disease (IBD) that predominantly affects the gastrointestinal tract. It is marked by...
01:17Chronic Bowel Disorders: Introduction
Irritable Bowel Syndrome (IBS) is a common disorder affecting the gastrointestinal tract. The distinctive feature is recurrent abdominal pain associated with altered bowel movements, manifesting as constipation, diarrhea, or fluctuating between both. The...
01:29Drugs for Treatment of Crohn's Disease in IBD Using Immunomodulatory Agents
01:27Inflammatory Bowel Disease I: Ulcerative Colitis
Inflammatory bowel disease, or IBD, encompasses a group of disorders characterized by chronic inflammation or ulceration of the gastrointestinal tract.
Risk Factors
The exact cause of IBD remains unclear, although it is believed to be due to a mix of genetic, environmental, microbial, and immune factors. Genetic factors are significant in determining susceptibility to IBD, with family history being a critical risk factor. Individuals with a first-degree relative who has IBD are at...
01:24Drugs for Treatment of Crohn's Disease in IBD Using Biologic Agents: Anti-TNF
01:30Inflammatory Bowel Disease III: Diagnostic Studies and Management I-Nutritional Therapy
Diagnostic studies
A colonoscopy is the definitive screening test, distinguishing ulcerative colitis from other colon diseases with similar symptoms. During a colonoscopy test, inflamed mucosa with exudate ulcerations can be observed, and biopsies are taken to determine the histologic characteristics of the...