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En Face Detection of Nitric Oxide and Superoxide in Endothelial Layer of Intact Arteries
Published on: February 25, 2016
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786T/c endothelial nitric oxide synthase gene polymorphism and coronary collateral circulation
Satilmis Seckin1, Bozbeyoglu Emrah2, Ismail Biyik3
1Department of Cardiology, Acıbadem University, School of Medicine, Atakent Hospital, Istanbul, Turkey.
Postepy Higieny I Medycyny Doswiadczalnej (Online)
|February 12, 2016
Summary
The NOS3 gene -786T/C polymorphism is linked to poor coronary collateral circulation (CCC) development in stable coronary artery disease patients. This genetic variation may impact angiogenesis and CCC formation.
Area of Science:
- Genetics
- Cardiovascular Medicine
- Molecular Biology
Background:
- The endothelial nitric oxide synthase (NOS3) gene plays a crucial role in vascular function.
- The -786T/C polymorphism (rs 2070744) in the NOS3 gene promoter region has been investigated for its potential impact on gene expression.
- Coronary collateral circulation (CCC) is a significant determinant of outcomes in patients with coronary artery disease.
Purpose of the Study:
- To investigate the association between the -786T/C polymorphism of the NOS3 gene and the development of coronary collateral circulation (CCC) in patients with stable coronary artery disease.
- To determine if specific genotypes of the NOS3 -786T/C polymorphism correlate with the quality of CCC.
Main Methods:
- Genotyping of the NOS3 -786T/C polymorphism using the polymerase chain reaction method.
- Classification of CCC based on the Rentrop classification, categorizing patients into poor (R0-1) and good (R2-3) CCC groups.
- Statistical analysis including chi-square tests and multivariate regression analysis to assess the association between genotypes and CCC status.
Main Results:
- A higher frequency of cytosine-cytosine (CC) and thymidine-cytosine (TC) genotypes and the C allele was observed in the poor CCC group, though not statistically significant.
- The dominant model (CC+TC genotypes) was significantly more prevalent in the poor CCC group compared to the thymidine-thymidine (TT) genotype group (67.1% vs. 54.5%, p=0.02).
- Multivariate regression analysis identified the dominant model for the NOS3 -786T/C polymorphism as an independent correlate of poor CCC.
Conclusions:
- The -786T/C polymorphism of the NOS3 gene (rs 2070744) is potentially associated with impaired angiogenesis.
- This genetic variation may influence the development of coronary collateral circulation in individuals with stable coronary artery disease.
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