Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Necrosis01:16

Necrosis

7.2K
Necrosis is considered as an “accidental” or unexpected form of cell death that ends in cell lysis. The first noticeable mention of “necrosis” was in 1859 when Rudolf Virchow used this term to describe advanced tissue breakdown in his compilation titled “Cell Pathology”.
Morphological Manifestations of Necrosis
Necrotic cells show different types of morphological appearance depending on the type of tissue and infection. In coagulative necrosis, cells become...
7.2K
Phagocytosis of Apoptotic Cells01:17

Phagocytosis of Apoptotic Cells

5.5K
Cells undergoing apoptosis form apoptotic bodies that must be removed immediately to prevent inflammation, autoimmune diseases, and necrosis. Phagocytosis is carried out by professional phagocytes such as macrophages or  immature dendritic cells. Non-professional phagocytes such as  epithelial cells and fibroblasts also take part in this process; however, they are not as effective as professional phagocytes. 
Normal cells contain receptors that prevent them from being recognized...
5.5K
Inflammation01:38

Inflammation

64.1K
Overview
64.1K
Overview of Cell Death01:30

Overview of Cell Death

11.0K
Cell death is an essential process where the body gets rid of old or damaged cells. Cell proliferation and death need to be balanced, as an imbalance between the two may lead to cancer or autoimmune diseases.
Cell death was observed in the early 19th century, but there was no experimental evidence to prove it. In 1842, Carl Vogt first discovered cell death in a metamorphic toad; however, it was not termed ‘cell death.’ Scientists discovered different cell death pathways only in the...
11.0K
Autophagic Cell Death01:18

Autophagic Cell Death

5.0K
Christian de Duve discovered “autophagy,” a process in which cellular components are engulfed by membrane-bound organelles called autophagosomes. The autophagosomes then fuse with lysosomes to digest the enclosed contents. Autophagy is generally activated in cells to prevent cell death. However, cell death is triggered when the damage is beyond repair.
Autophagy and Apoptosis
Autophagy can activate apoptosis. In normal conditions, the autophagy activating protein Beclin-1 and...
5.0K
Apoptosis01:30

Apoptosis

16.8K
Apoptosis is a combination of two Greek words, 'apo' and 'ptosis,' meaning separation and falling off, respectively. Hippocrates used this word to describe gangrene, which was caused due to bandaging of fractured bones. Apoptosis was distinguished from necrosis in 1970 when John Kerr reported observations of morphological changes occurring during apoptosis. During one experiment, he observed that the disruption of blood supply to the liver tissue resulted in a size...
16.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Protein kinase C theta: evolution, regulation, and function.

The Biochemical journal·2026
Same author

Ubiquitin Ligase COP1 Suppresses Neuroinflammation by Degrading c/EBPβ in Microglia.

Cell·2026
Same author

cFLIP suppresses caspase-1- and MLKL-independent perinatal lethality driven by auto-processing impaired caspase-8 D387A.

Cell death and differentiation·2025
Same author

Author Correction: Activity of caspase-8 determines plasticity between cell death pathways.

Nature·2025
Same author

Author Correction: Cleavage of RIPK1 by caspase-8 is crucial for limiting apoptosis and necroptosis.

Nature·2025
Same author

Author Correction: Inhibiting membrane rupture with NINJ1 antibodies limits tissue injury.

Nature·2025
Same journal

Lactate as a Chemical Modification on Proteins and Metabolites.

Annual review of biochemistry·2026
Same journal

Nucleocytoplasmic Transport.

Annual review of biochemistry·2026
Same journal

Packaging of Single-Stranded RNA in Viruses and Virus-Like Particles.

Annual review of biochemistry·2026
Same journal

Shaping of the Infant Gut Microbiome by Milk Oligosaccharides.

Annual review of biochemistry·2026
Same journal

Proteostasis Deregulation by Metabolism Drives the Hallmarks of Cancer.

Annual review of biochemistry·2026
Same journal

JoAnne Stubbe's Radical Path: A Story of Passion, Curiosity, and Persistence.

Annual review of biochemistry·2026
See all related articles

Related Experiment Video

Updated: Mar 26, 2026

Evaluation of Caspase Activation to Assess Innate Immune Cell Death
10:23

Evaluation of Caspase Activation to Assess Innate Immune Cell Death

Published on: January 20, 2023

3.9K

Necroptosis and Inflammation.

Kim Newton1, Gerard Manning2

  • 1Physiological Chemistry Department, Genentech, Inc., South San Francisco, California 94080;

Annual Review of Biochemistry
|February 12, 2016
PubMed
Summary
This summary is machine-generated.

Necroptosis, a regulated cell death, triggers immune responses when caspase-8 is inhibited. Targeting RIPK1 or RIPK3 kinases may offer therapeutic benefits for inflammatory diseases.

Keywords:
MLKLRIPK1RIPK3

More Related Videos

Characterization of MLKL-mediated Plasma Membrane Rupture in Necroptosis
08:55

Characterization of MLKL-mediated Plasma Membrane Rupture in Necroptosis

Published on: August 7, 2018

11.4K
Identification of Intracellular Signaling Events Induced in Viable Cells by Interaction with Neighboring Cells Undergoing Apoptotic Cell Death
09:18

Identification of Intracellular Signaling Events Induced in Viable Cells by Interaction with Neighboring Cells Undergoing Apoptotic Cell Death

Published on: December 27, 2016

9.2K

Related Experiment Videos

Last Updated: Mar 26, 2026

Evaluation of Caspase Activation to Assess Innate Immune Cell Death
10:23

Evaluation of Caspase Activation to Assess Innate Immune Cell Death

Published on: January 20, 2023

3.9K
Characterization of MLKL-mediated Plasma Membrane Rupture in Necroptosis
08:55

Characterization of MLKL-mediated Plasma Membrane Rupture in Necroptosis

Published on: August 7, 2018

11.4K
Identification of Intracellular Signaling Events Induced in Viable Cells by Interaction with Neighboring Cells Undergoing Apoptotic Cell Death
09:18

Identification of Intracellular Signaling Events Induced in Viable Cells by Interaction with Neighboring Cells Undergoing Apoptotic Cell Death

Published on: December 27, 2016

9.2K

Area of Science:

  • Cellular Biology
  • Immunology
  • Molecular Medicine

Background:

  • Necroptosis is a regulated form of necrosis.
  • It involves cell rupture and release of intracellular components, initiating innate immune responses.
  • Triggers include TLR agonists, TNF, viral infections, or TCR activation when caspase-8 is inhibited.

Purpose of the Study:

  • To explore the role of RIPK1 and RIPK3 kinases in necroptosis.
  • To investigate the therapeutic potential of inhibiting these kinases.

Main Methods:

  • Analysis of necroptosis signaling pathways.
  • Evaluation of RIPK3 deficiency and RIPK1 inhibition in disease models.

Main Results:

  • Necroptosis signaling requires RIPK3 and MLKL, modulated by RIPK1.
  • RIPK3 deficiency or RIPK1 inhibition confers resistance in animal models.
  • Inflammation from necroptosis contributes to tissue damage.

Conclusions:

  • RIPK1 and RIPK3 are key regulators of necroptosis and inflammation.
  • Inhibitors of RIPK1 or RIPK3 show therapeutic potential for inflammatory conditions.
  • These kinases may regulate cell death programs beyond necroptosis.