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Modulation of gene expression by auxin.

J L Key

    Bioessays : News and Reviews in Molecular, Cellular and Developmental Biology
    |August 1, 1989
    PubMed
    Summary

    Plant hormone auxin rapidly alters gene expression, affecting mRNA levels through transcription modulation and turnover. This suggests a direct link between auxin

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    Area of Science:

    • Plant biology
    • Molecular biology
    • Genetics

    Background:

    • Auxin is a critical plant hormone regulating growth and development.
    • Auxin influences diverse processes like cell elongation and division.
    • Gene expression changes rapidly and dramatically in response to auxin.

    Purpose of the Study:

    • To investigate the rapid and selective alterations in gene expression induced by auxin.
    • To explore the mechanisms underlying auxin's effects on mRNA levels.
    • To assess the relationship between auxin-regulated gene expression and plant growth responses.

    Main Methods:

    • Quantification of mRNA steady-state levels using cloned cDNAs.
    • Measurement of relative transcription rates via run-off transcription assays.
    • Isolation of nuclei from control and auxin-treated plant tissues.

    Main Results:

    • Auxin rapidly modulates transcription (within 5-10 min) in plant tissues.
    • Significant increases and decreases in specific mRNA levels were observed.
    • Rapid mRNA turnover contributes to changes in steady-state concentrations.

    Conclusions:

    • Auxin employs multiple, complex mechanisms to regulate gene expression.
    • Evidence strongly supports a close relationship between auxin's effects on gene expression and physiological responses.
    • A causal link between auxin-induced gene expression changes and growth responses is hypothesized.

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