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Plant Inoculation with the Fungal Leaf Pathogen Colletotrichum higginsianum
Kei Hiruma1, Yusuke Saijo2,3
1Graduate School of Biological Sciences, Nara Institute of Science and Technology, Takayama-cho 8916-5, Ikoma, Nara, 630-0192, Japan.
Methods in Molecular Biology (Clifton, N.J.)
|February 13, 2016
Abstract:
After Colletotrichum storage methods in Chapter 23 , we describe here experimental methods for the inoculation of Colletotrichum higginsianum (C.h.) on Arabidopsis leaves. We put a particular focus on the methods for lesion measurements after the drop-inoculation of the leaves and on C.h. biomass measurements in the leaves by RT-qPCR analysis. As an option, we also briefly describe methods for counting C.h. entry ratio.
