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Rapid Assessment of DNA Methylation Changes in Response to Salicylic Acid by Chop-qPCR
Stephanie Rausch1,2,3, Sascha Laubinger4,5,6
1Center for Plant Molecular Biology (ZMBP), University of Tübingen, 72076, Tübingen, Germany.
Methods in Molecular Biology (Clifton, N.J.)
|February 13, 2016
Abstract:
Methylation of cytosines plays an important role in epigenetic regulation of gene expression. Several methods exist to determine the methylation status of DNA. Here, we describe a rapid and cost-effective method called Chop-qPCR to determine dynamic changes in the DNA methylation patterns, as they occur for instance in response to environmental stresses.
