Dermabacter vaginalis sp. nov., isolated from human vaginal fluid

Area of Science:

• Microbiology
• Bacteriology
• Taxonomy

Background:

• Actinobacteria are a diverse phylum of Gram-positive bacteria.
• The human vaginal microbiome plays a crucial role in health and disease.
• Novel bacterial species identification contributes to understanding microbial ecosystems.

Purpose of the Study:

• To characterize a novel actinobacterial strain, AD1-86T, isolated from vaginal fluid.
• To determine the taxonomic placement of strain AD1-86T within the genus Dermabacter.
• To propose a new species designation for the novel isolate.

Main Methods:

• Polyphasic characterization including phenotypic, chemotaxonomic, and genotypic analyses.
• 16S rRNA gene sequencing for phylogenetic analysis.
• DNA-DNA hybridization for assessing genomic relatedness.

Main Results:

• Strain AD1-86T is a facultatively anaerobic, Gram-positive, non-spore-forming rod.
• Phylogenetic analysis showed highest similarity to Dermabacter hominis (98.3%).
• DNA-DNA relatedness to D. hominis DSM 7083T was 31.1±3.0%, supporting a new species designation.

Conclusions:

• Strain AD1-86T represents a novel species of the genus Dermabacter.
• The proposed name for this new species is Dermabacter vaginalis sp. nov.
• The type strain is AD1-86T (=KCTC 39585T=DSM 100050T).