Related Experiment Video
Updated: Mar 25, 2026
09:04
Transcriptome Profiling of In-Vivo Produced Bovine Pre-implantation Embryos Using Two-color Microarray Platform
Published on: January 30, 2017
8.2K
Chicken sperm transcriptome profiling by microarray analysis
R P Singh1, C M Shafeeque1, S K Sharma2
1a Avian Physiology and Genetics Division, Sálim Ali Centre for Ornithology and Natural History, Anaikatty-641108, Coimbatore, India.
Genome
|February 13, 2016
Summary
Chicken sperm contain thousands of functional RNAs, with many unique transcripts not found in testes. These sperm RNAs are crucial for fertilization and early embryonic development, offering potential fertility biomarkers.
Area of Science:
- Reproductive Biology
- Genomics
- Molecular Biology
Background:
- Mammalian sperm possess functional RNAs vital for fertilization and embryonic development.
- Understanding avian sperm transcriptomes is essential for reproductive research.
Purpose of the Study:
- To characterize the transcriptome of fertile chicken sperm using microarray analysis.
- To identify sperm-specific RNA transcripts and their potential functions.
Main Methods:
- Sperm RNA was extracted from 10 fertile chickens and analyzed using a 4 × 44K chicken microarray.
- RNA quality and contamination were assessed using a bioanalyzer and gene amplification (CD4, PTPRC).
- Results were validated by RT-PCR, comparing sperm and testes RNA.
Main Results:
- Identified 21,639 predominantly nuclear-encoded transcripts in chicken sperm.
- 33.45% of transcripts were unique to sperm, not detected in testes.
- Key transcript functions included signal transduction, embryonic development, and cell structure.
Conclusions:
- Chicken sperm possess a unique and complex transcriptome.
- Sperm-specific transcripts may play critical roles in avian reproduction.
- These findings provide a basis for identifying sperm-based fertility biomarkers in chickens.
Related Concept Videos
02:34DNA Microarrays
22.8K
Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
22.8K
02:24Ribosome Profiling
4.3K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
4.3K