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Related Experiment Video

Updated: Mar 25, 2026

Transcriptome Profiling of In-Vivo Produced Bovine Pre-implantation Embryos Using Two-color Microarray Platform

Transcriptome Profiling of In-Vivo Produced Bovine Pre-implantation Embryos Using Two-color Microarray Platform

Published on: January 30, 2017

Chicken sperm transcriptome profiling by microarray analysis.

R P Singh¹, C M Shafeeque¹, S K Sharma²

¹a Avian Physiology and Genetics Division, Sálim Ali Centre for Ornithology and Natural History, Anaikatty-641108, Coimbatore, India.

|February 13, 2016

Summary

This summary is machine-generated.

Chicken sperm contain thousands of functional RNAs, with many unique transcripts not found in testes. These sperm RNAs are crucial for fertilization and early embryonic development, offering potential fertility biomarkers.

Keywords:

ARN de sperme biomarqueur pour la fécondité développement embyronnaire egg embryonic development fertility biomarker fertilization fécondation sperm RNA œuf

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Area of Science:

Reproductive Biology
Genomics
Molecular Biology

Background:

Mammalian sperm possess functional RNAs vital for fertilization and embryonic development.
Understanding avian sperm transcriptomes is essential for reproductive research.

Purpose of the Study:

To characterize the transcriptome of fertile chicken sperm using microarray analysis.
To identify sperm-specific RNA transcripts and their potential functions.

Main Methods:

Sperm RNA was extracted from 10 fertile chickens and analyzed using a 4 × 44K chicken microarray.
RNA quality and contamination were assessed using a bioanalyzer and gene amplification (CD4, PTPRC).
Results were validated by RT-PCR, comparing sperm and testes RNA.

Main Results:

Identified 21,639 predominantly nuclear-encoded transcripts in chicken sperm.
33.45% of transcripts were unique to sperm, not detected in testes.
Key transcript functions included signal transduction, embryonic development, and cell structure.

Conclusions:

Chicken sperm possess a unique and complex transcriptome.
Sperm-specific transcripts may play critical roles in avian reproduction.
These findings provide a basis for identifying sperm-based fertility biomarkers in chickens.