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Updated: Aug 10, 2026
Isolation and Activation of Murine Lymphocytes
Published on: October 30, 2016
Effect of dexamethasone on the expression of interleukin-2 in a mouse T cell line
B A Sorg1, N S Magnuson, R Reeves
1Department of Genetics and Cell Biology, Washington State University, Pullman 99164.
1. The effect of the synthetic glucocorticoid, dexamethasone (dex), on the production of interleukin-2 (IL-2) and IL-2 mRNA was examined in the mouse T cell line, LBRM-33.4A2. 2. Treatment of Concanavalin A (Con A)-stimulated LBRM-33 cells with low concentrations of dex (10nM) inhibited the production of IL-2 activity by approx. 70%, with a corresponding decrease in IL-2 mRNA levels. 3. In contrast, much higher concentrations of dex (up to 10 microM) inhibited the level of another Con A-inducible mRNA, c-myc mRNA, by only 30%, and did not affect beta-tubulin mRNA levels at all. 4. Thus it appears that inhibition by dex in stimulated LBRM-33 cells is specific for the expression of IL-2 mRNA. 5. Experiments with actinomycin D suggest that dex does not mediate its suppression of IL-2 mRNA accumulation by decreasing the stability of this message; rather, it appears that dex inhibits transcription of the IL-2 gene.
1. The effect of the synthetic glucocorticoid, dexamethasone (dex), on the production of interleukin-2 (IL-2) and IL-2 mRNA was examined in the mouse T cell line, LBRM-33.4A2. 2. Treatment of Concanavalin A (Con A)-stimulated LBRM-33 cells with low concentrations of dex (10nM) inhibited the production of IL-2 activity by approx. 70%, with a corresponding decrease in IL-2 mRNA levels. 3. In contrast, much higher concentrations of dex (up to 10 microM) inhibited the level of another Con A-inducible mRNA, c-myc mRNA, by only 30%, and did not affect beta-tubulin mRNA levels at all. 4. Thus it appears that inhibition by dex in stimulated LBRM-33 cells is specific for the expression of IL-2 mRNA. 5. Experiments with actinomycin D suggest that dex does not mediate its suppression of IL-2 mRNA accumulation by decreasing the stability of this message; rather, it appears that dex inhibits transcription of the IL-2 gene.
