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Constitutive expression of a native human interferon-alpha 1 gene in Escherichia coli
I Ivanov1, N Markova, D Bachvarov
1Institute of Molecular Biology, Bulgarian Academy of Sciences, Sofia.
The International Journal of Biochemistry
|January 1, 1989
Abstract:
1. A plasmid for constitutive expression of the human interferon-alpha 1 (hIFN-alpha 1) gene in Escherichia coli is constructed on the basis of the cloning plasmid pBR322 using a strong synthetic promoter, synthetic ribosome binding site and a native hIFN-alpha 1 gene excised from a chromosomal clone. 2. The yield of recombinant hIFN-alpha 1 from E. coli LE392 cells transformed with the expression plasmid pJP1R9-hIFN-alpha 1 is evaluated to be 2-6 x 10(7) U/l bacterial culture for metabolic shaker and 6-8 x 10(7) U/l for fermentor.