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Synthesis and secretion of human nerve growth factor by Saccharomyces cerevisiae
E Kanaya1, T Higashizaki, F Ozawa
1Mitsubishi Kasei Institute of Life Sciences, Tokyo, Japan.
Gene
|November 15, 1989
Abstract:
The DNA coding for human nerve growth factor (hNGF) was chemically synthesized and introduced into Saccharomyces cerevisiae. Expression and secretion of hNGF was obtained by use of the yeast phosphoglycerate kinase-encoding gene promoter and the pre-pro sequence of the yeast alpha-mating factor. Immunoblotting with antiserum raised against a protein A-hNGF fusion protein, allowed the detection of an immunoreactive material secreted into the culture medium. A preparation from the culture medium, partially purified by ion-exchange column chromatography, stimulated neurite outgrowth from rat pheochromocytoma PC12h cells.