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Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

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Published on: July 5, 2016

Swept source digital holographic phase microscopy.

Shichao Chen, Junghyun Ryu, Kiho Lee

|February 13, 2016

Summary

This summary is machine-generated.

This study introduces a digital holographic phase microscopy method using a swept source laser. It achieves high pathlength sensitivity for imaging live sperm cells and retrieving spectroscopic information.

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Area of Science:

Optical microscopy
Holography
Spectroscopy

Background:

Digital holographic microscopy (DHM) is a powerful technique for quantitative phase imaging.
Traditional DHM can suffer from interference artifacts, limiting accuracy.
Spectral domain interferometry offers a way to improve phase determination.

Purpose of the Study:

To develop a swept source-based digital holographic phase microscopy technique.
To achieve high pathlength sensitivity and reduce artifacts in holographic imaging.
To enable simultaneous phase and spectroscopic information retrieval from samples.

Main Methods:

Utilizing a swept source laser to acquire a series of on-axis interferograms.
Applying spectral domain interferometry for accurate sample phase determination.
Identifying and avoiding interference artifacts by analyzing sample spectra at clean spectral frequencies.

Main Results:

Achieved pathlength sensitivity better than 0.3 nm.
Obtained quantitative pathlength images of live sperm cells with clear morphological feature identification.
Retrieved wavelength-dependent refractive indices of indocyanine green solution, demonstrating spectroscopic capability.

Conclusions:

The proposed swept source DHM technique effectively reduces artifacts and enhances phase imaging accuracy.
This method allows for detailed morphological analysis of biological samples like sperm cells.
The technique provides a platform for multimodal imaging, combining phase contrast and spectroscopy.