Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Electrospray Ionization (ESI) Mass Spectrometry01:12

Electrospray Ionization (ESI) Mass Spectrometry

2.7K
Higher molecular weight biomolecules are nonvolatile compounds that may decompose before ionizing or vaporizing during mass analysis with conventional electron impact ionization methods. Accordingly, electrospray ionization (ESI) is the favored method for vaporizing and ionizing biomolecules as it circumvents rapid fragmentation and enables the recording of mass signals for the entire biomolecule.
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...
2.7K
Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

1.7K
Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
1.7K
Capillary Electrophoresis: Instrumentation01:20

Capillary Electrophoresis: Instrumentation

1.6K
Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
1.6K
Electrophoresis: Overview01:20

Electrophoresis: Overview

4.9K
Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...
4.9K
Ion-Exchange Chromatography01:09

Ion-Exchange Chromatography

2.8K
Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...
2.8K
High-Performance Liquid Chromatography: Types of Detectors01:15

High-Performance Liquid Chromatography: Types of Detectors

2.1K
The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte...
2.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

In-situ product recovery in microfluidic bioreactors.

Current opinion in biotechnology·2026
Same author

Development of an ultra-fast polarity-switching ion mobility spectrometer ready for coupling with supercritical fluid chromatography.

Journal of chromatography. A·2025
Same author

Fast Chemical Analysis of Droplets Unlocked by Ultra-Fast Ion Mobility Spectrometry.

Analytical chemistry·2025
Same author

A Microfluidic Device to Realize Electrochemically Controlled SERS Detection in HPLC.

Analytical chemistry·2025
Same author

High-spatial-resolution mass spectrometry imaging of biological tissues using a microfluidic probe.

Nature protocols·2025
Same author

Coupling Capillary Electrophoresis With a Shifted Inlet Potential High-Resolution Ion Mobility Spectrometer.

Electrophoresis·2025

Related Experiment Video

Updated: Mar 25, 2026

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis
14:53

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

Published on: September 10, 2014

17.9K

Chip-based electrochromatography coupled to ESI-MS detection.

Claudia Dietze1, Claudia Hackl1, Renata Gerhardt1

  • 1Institute of Analytical Chemistry, University of Leipzig, Leipzig, Germany.

Electrophoresis
|February 14, 2016
PubMed
Summary

This study couples chip-based electrochromatography to mass spectrometry (MS) using a novel integrated emitter. The method enables high-speed drug analysis, demonstrating its potential for pharmaceutical applications.

Keywords:
ElectrochromatographyMass spectrometryMicrochip

More Related Videos

Capillary Electrophoresis Mass Spectrometry Approaches for Characterization of the Protein and Metabolite Corona Acquired by Nanomaterials
07:54

Capillary Electrophoresis Mass Spectrometry Approaches for Characterization of the Protein and Metabolite Corona Acquired by Nanomaterials

Published on: October 27, 2020

5.0K
Sheathless Capillary Electrophoresis–Mass Spectrometry for Metabolic Profiling of Biological Samples
07:46

Sheathless Capillary Electrophoresis–Mass Spectrometry for Metabolic Profiling of Biological Samples

Published on: October 1, 2016

12.3K

Related Experiment Videos

Last Updated: Mar 25, 2026

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis
14:53

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

Published on: September 10, 2014

17.9K
Capillary Electrophoresis Mass Spectrometry Approaches for Characterization of the Protein and Metabolite Corona Acquired by Nanomaterials
07:54

Capillary Electrophoresis Mass Spectrometry Approaches for Characterization of the Protein and Metabolite Corona Acquired by Nanomaterials

Published on: October 27, 2020

5.0K
Sheathless Capillary Electrophoresis–Mass Spectrometry for Metabolic Profiling of Biological Samples
07:46

Sheathless Capillary Electrophoresis–Mass Spectrometry for Metabolic Profiling of Biological Samples

Published on: October 1, 2016

12.3K

Area of Science:

  • Analytical Chemistry
  • Separation Science
  • Mass Spectrometry

Background:

  • Chip-based separations offer miniaturization and efficiency.
  • Integrating electrochromatography with mass spectrometry is challenging.
  • Nanoelectrospray ionization is crucial for interfacing microscale devices with MS.

Purpose of the Study:

  • To develop and validate a chip-based electrochromatography system coupled to mass spectrometry.
  • To demonstrate the utility of this system for rapid drug analysis.

Main Methods:

  • Fabrication of a glass chip with a monolithically integrated nanoelectrospray emitter.
  • Implementation of an acrylate-based porous polymer monolith as the separation column via photopolymerization.
  • Utilizing a test mixture for method development and validation, detectable by fluorescence and ESI-MS.

Main Results:

  • Successful coupling of chip-based electrochromatography to MS was achieved.
  • The developed method demonstrated high-speed separation capabilities.
  • Exemplary application for the analysis of benzodiazepines in pharmaceutical samples was successful.

Conclusions:

  • The integrated chip-based electrochromatography-MS system is a viable tool for rapid and efficient separation and analysis.
  • This technology holds promise for pharmaceutical analysis and other applications requiring high-throughput screening.