Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Proteomics01:33

Proteomics

10.1K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
10.1K
Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

8.8K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
8.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

PepMapViz: a versatile toolkit for peptide mapping, visualization, and comparative exploration.

Bioinformatics (Oxford, England)·2025
Same author

Comprehensive Protein Inference Analysis with PyProteinInference Elucidates Biological Understanding of Tandem Mass Spectrometry Data.

Journal of proteome research·2025
Same author

UniDec Processing Pipeline for Rapid Analysis of Biotherapeutic Mass Spectrometry Data.

Analytical chemistry·2023
Same author

Complement C1q-dependent excitatory and inhibitory synapse elimination by astrocytes and microglia in Alzheimer's disease mouse models.

Nature aging·2023
Same author

Discovery of a caspase cleavage motif antibody reveals insights into noncanonical inflammasome function.

Proceedings of the National Academy of Sciences of the United States of America·2021
Same author

Monitoring protein communities and their responses to therapeutics.

Nature reviews. Drug discovery·2020
Same journal

Platelet proteome links metabolism to reactivity in Essential Thrombocythemia.

Molecular & cellular proteomics : MCP·2026
Same journal

Genetic rescue of disrupted synaptic protein interaction network dynamics following SYNGAP1 reactivation.

Molecular & cellular proteomics : MCP·2026
Same journal

ASAP-ID: Proximity labelling with small tags.

Molecular & cellular proteomics : MCP·2026
Same journal

Proteome profiling reveals NQO2 activity contributing to proteasome inhibitor resistance in multiple myeloma cell lines.

Molecular & cellular proteomics : MCP·2026
Same journal

Depletion-Free Automated Enrichment of Serum Glycopeptides for High-Throughput Clinical Glycoproteomics.

Molecular & cellular proteomics : MCP·2026
Same journal

Extracellular Vesicles from Glioblastoma Cells Reflect 2D vs. 3D Culture Adaptation and Resistance to Temozolomide.

Molecular & cellular proteomics : MCP·2026
See all related articles

Related Experiment Video

Updated: Mar 25, 2026

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

12.9K

A Biologist's Field Guide to Multiplexed Quantitative Proteomics.

Corey E Bakalarski1, Donald S Kirkpatrick2

  • 1From the Departments of ‡Protein Chemistry and §Bioinformatics and Computational Biology, Genentech, Inc., South San Francisco, California 94080.

Molecular & Cellular Proteomics : MCP
|February 14, 2016
PubMed
Summary
This summary is machine-generated.

Quantitative proteomics offers dynamic biological insights beyond static genomic catalogs. This review guides biologists in selecting mass spectrometry methods for meaningful data and mechanistic understanding.

More Related Videos

Enhanced Sample Multiplexing of Tissues Using Combined Precursor Isotopic Labeling and Isobaric Tagging cPILOT
09:06

Enhanced Sample Multiplexing of Tissues Using Combined Precursor Isotopic Labeling and Isobaric Tagging cPILOT

Published on: May 1, 2017

7.5K
Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization
12:11

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization

Published on: February 27, 2020

7.4K

Related Experiment Videos

Last Updated: Mar 25, 2026

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

12.9K
Enhanced Sample Multiplexing of Tissues Using Combined Precursor Isotopic Labeling and Isobaric Tagging cPILOT
09:06

Enhanced Sample Multiplexing of Tissues Using Combined Precursor Isotopic Labeling and Isobaric Tagging cPILOT

Published on: May 1, 2017

7.5K
Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization
12:11

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization

Published on: February 27, 2020

7.4K

Area of Science:

  • Proteomics
  • Mass Spectrometry
  • Systems Biology

Background:

  • High-throughput omics studies provide static biological catalogs.
  • Static data is insufficient for understanding dynamic biological processes.
  • Quantitative proteomics reveals insights into dynamic biological states.

Purpose of the Study:

  • To outline quantitative proteomic mass spectrometry methods.
  • To discuss the benefits and weaknesses of these methods.
  • To aid biologists in selecting appropriate tools for meaningful data generation.

Main Methods:

  • Review of various quantitative proteomic mass spectrometry technologies.
  • Analysis of strengths and weaknesses of different methods.
  • Perspective tailored for biologists addressing mechanistic questions.

Main Results:

  • Quantitative proteomics enables the study of dynamic biological processes.
  • A variety of quantitative proteomic techniques exist, each with pros and cons.
  • Guidance is provided for selecting the optimal method based on experimental needs.

Conclusions:

  • Understanding quantitative proteomics methods is crucial for biologists.
  • Method selection impacts the quality and relevance of proteomic data.
  • This review facilitates informed choices for advancing biological research.