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Effects of receptor for advanced glycation endproducts on microvessel formation in endometrial cancer
Lu Zheng1,2, Da Li3, Yi-Ming Zhou4
1Department of Obstetrics and Gynecology, Shengjing Hospital of China Medical University, Shenyang, 110004, China. zhenglu1980@yeah.net.
Background:
The receptor for advanced glycation endproducts (RAGE) and microvascular status both play a critical role in cancer progression. However, the crosstalk between RAGE and microvascular formation in endometrial cancer remains largely unknown.
Methods:
RAGE expression and microvessel density were examined in 20 cases of normal endometrial tissue, 37 cases of well-differentiated endometrial cancer tissue, and 35 cases of poorly-differentiated endometrial cancer tissue. Regression analysis was used to examine the relationship between RAGE and microvessel density. The knockdown of RAGE was achieved using a small interfering RNA in HEC-1A endometrial cancer cells. A xenografted tumour model was used to evaluate RAGE-mediated microvascular formation and proliferation of endometrial cancer cells.
Results:
It was shown that (i) RAGE expression gradually increased in normal endometrium, well-differentiated endometrial cancer, and poorly-differentiated endometrial cancer, respectively; (ii) a positive correlation existed between RAGE and microvessel density in human endometrial cancer samples; (iii) RAGE knockdown was effective in decreasing microvessel formation in xenografted tumour models; and (iv) RAGE knockdown can significantly inhibit the proliferation of endometrial cancer cells in vivo.
Conclusions:
These results indicate that RAGE may be a potential trigger in microvascular formation and proliferation in the development of endometrial cancer.
Insights
The receptor for advanced glycation endproducts (RAGE) is linked to increased microvascular formation and cancer cell proliferation in endometrial cancer. Targeting RAGE may inhibit tumor growth by reducing blood vessel development.
Area of Science:
- Oncology
- Vascular Biology
- Molecular Biology
Background:
- The receptor for advanced glycation endproducts (RAGE) and microvascular status are crucial in cancer progression.
- The interaction between RAGE and microvascular formation in endometrial cancer is not well understood.
Purpose of the Study:
- To investigate the role of RAGE in microvascular formation and endometrial cancer cell proliferation.
- To determine the correlation between RAGE expression and microvessel density in endometrial cancer.
Main Methods:
- Examined RAGE expression and microvessel density in normal and cancerous endometrial tissues.
- Utilized regression analysis to assess the RAGE-microvessel density relationship.
- Performed RAGE knockdown in endometrial cancer cells and evaluated its effect in a xenograft model.
Main Results:
- RAGE expression increased progressively from normal endometrium to poorly-differentiated endometrial cancer.
- A positive correlation was observed between RAGE expression and microvessel density.
- RAGE knockdown reduced microvessel formation and significantly inhibited endometrial cancer cell proliferation in vivo.
Conclusions:
- RAGE appears to be a key factor in promoting microvascular formation and proliferation in endometrial cancer.
- RAGE represents a potential therapeutic target for managing endometrial cancer progression.
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