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Published on: June 18, 2020
Probing the nucleotide-binding activity of a redox sensor: two-component regulatory control in chloroplasts
Iskander M Ibrahim1, Sujith Puthiyaveetil2, Christine Khan3
1Faculty of Engineering and Science, University of Greenwich, Chatham Maritime, Kent, UK.
Abstract:
Two-component signal transduction systems mediate adaptation to environmental changes in bacteria, plants, fungi, and protists. Each two-component system consists of a sensor histidine kinase and a response regulator. Chloroplast sensor kinase (CSK) is a modified sensor histidine kinase found in chloroplasts-photosynthetic organelles of plants and algae. CSK regulates the transcription of chloroplast genes in response to changes in photosynthetic electron transport. In this study, the full-length and truncated forms of Arabidopsis CSK proteins were overexpressed and purified in order to characterise their kinase and redox sensing activities. Our results show that CSK contains a modified kinase catalytic domain that binds ATP with high affinity and forms a quinone adduct that may confer redox sensing activity.
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