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MicroRNA-183 functions as the tumor suppressor via inhibiting cellular invasion and metastasis by targeting MMP-9 in

Dongmei Fan1, Ying Wang2, Pengwei Qi1

  • 1Gynaecology and Obstetrics Department of the First Affiliated Hospital of Henan University of Science and Technology, No. 24 Jinghua Road, Luoyang, Henan 471003, China.

Gynecologic Oncology
|February 14, 2016
PubMed
Abstract

Insights

MicroRNA-183 (miR-183) is downregulated in cervical cancer, inhibiting tumor cell invasion and metastasis. Targeting MMP-9, miR-183 acts as a tumor suppressor, offering a potential therapeutic strategy for cervical cancer.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • MicroRNAs regulate gene expression and are implicated in cancer progression.
  • The specific role of miR-183 in cervical cancer invasion and metastasis remains unclear.
  • miR-183 has shown varied effects in different solid tumors.

Purpose of the Study:

  • To investigate the expression levels of miR-183 in cervical cancer tissues.
  • To determine the functional role of miR-183 in the invasion and metastasis of cervical cancer cells.
  • To identify potential molecular targets of miR-183 in cervical cancer.

Main Methods:

  • Quantitative reverse transcription polymerase chain reaction (qRT-PCR) to measure miR-183 expression in 53 cervical cancer and 13 normal tissues.
  • Transwell assays (uncoated and Matrigel-coated) to assess cell migration and invasion after forced miR-183 expression.
  • Bioinformatics analysis and dual-luciferase reporter assays to identify and validate MMP-9 as a direct target of miR-183.

Main Results:

  • miR-183 expression was significantly downregulated in cervical cancer tissues compared to normal tissues.
  • Forced expression of miR-183 suppressed the invasion and migration of cervical cancer cell lines (siha and Hela).
  • MMP-9 was identified as a direct target of miR-183 and was found to be upregulated in cervical cancer; its downregulation inhibited cancer cell invasion and metastasis.

Conclusions:

  • miR-183 functions as a tumor suppressor in cervical cancer by inhibiting cell invasion and metastasis.
  • The tumor-suppressive role of miR-183 is mediated through the targeting of MMP-9.
  • miR-183 represents a potential novel therapeutic target for cervical cancer treatment.
Keywords:
Cervical cancerInvasionMMP-9MetastasismiR-183

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