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Updated: Mar 25, 2026

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Extracting conformational information from single molecule photon statistics.

Yonggang Peng¹, Chuanlu Yang², Yujun Zheng¹

¹School of Physics, Shandong University, Jinan 250100, China.

The Journal of Chemical Physics

|February 15, 2016

Summary

This study introduces resonant photon emission trajectories to reveal single-molecule conformational dynamics. The method accurately captures complex molecular behaviors, validated using Thioflavin T (ThT) molecules.

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Area of Science:

Physical Chemistry
Spectroscopy
Computational Chemistry

Background:

Understanding single-molecule conformational dynamics is crucial for complex biological systems.
Traditional methods often struggle with the intricacies of molecular motion in diverse environments.
Photon emission analysis offers a potential avenue for probing these dynamics.

Purpose of the Study:

To develop and validate a novel approach for extracting single-molecule conformational information.
To utilize resonant photon emission trajectories in conformational space and external field frequency.
To apply this method to understand the dynamics of single Thioflavin T (ThT) molecules.

Main Methods:

Employing resonant trajectories of photon emission (Traj〈N〉 and TrajQ) in conformational coordinate (X) and external field frequency (ωL) space.
Utilizing the Smoluchowski equation to model conformational dynamics in complex environments.
Applying the developed approach to single Thioflavin T (ThT) molecules.

Main Results:

The resonant trajectories method successfully extracts conformational information from single molecules.
The Smoluchowski equation effectively describes molecular dynamics in complex settings.
The approach demonstrated excellent agreement with ab initio simulation results for ThT.

Conclusions:

Resonant photon emission trajectories provide a powerful tool for single-molecule conformational analysis.
The method's accuracy is validated by its successful application to ThT and comparison with high-level simulations.
This technique offers new possibilities for studying molecular behavior in intricate environments.