Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Trihybrid Crosses02:27

Trihybrid Crosses

26.6K
Trihybrid Crosses
Some of Mendel’s crosses examined three pairs of contrasting characteristics. Such a cross is called a trihybrid cross. A trihybrid cross is a combination of three individual monohybrid crosses. For example, plant height (tall vs. short), seed shape (round vs. wrinkled), and seed color (yellow vs. green).
The F1 generation plants of a trihybrid cross are heterozygous for all three traits and produce eight gametes. Upon self-fertilization, these gametes have an equal...
26.6K
Cell Signaling in Plants01:25

Cell Signaling in Plants

7.0K
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
7.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Single-cell and spatial omics in plants: from cellular atlases to regulatory mechanisms.

Journal of experimental botany·2026
Same author

Unraveling plant phenotype to genotype associations with daily hyperspectral traits in <i>Populus trichocarpa</i>.

Plant phenomics (Washington, D.C.)·2026
Same author

Telomere-to-telomere assemblies of chromosome 10 reveal complex adaptive variation of 3-ketoacyl-CoA-synthases in <i>Populus trichocarpa</i> likely driven by Helitrons.

Forestry research·2026
Same author

High Terpene Production in Myrtaceae: Evolutionary Insights from Terpene Pathway Genes.

Plants (Basel, Switzerland)·2026
Same author

Increasing value in the Veterans Affairs Healthcare System (VA) with precision health: a continuing landmark collaboration with the Department of Energy.

Journal of the American Medical Informatics Association : JAMIA·2026
Same author

Higher Wood Density Lowers Feedstock Cost and Has Minimal Impact on Biomass Conversion to Biofuels.

ACS sustainable chemistry & engineering·2026

Related Experiment Video

Updated: Mar 25, 2026

The Use of Induced Somatic Sector Analysis ISSA for Studying Genes and Promoters Involved in Wood Formation and Secondary Stem Development
09:54

The Use of Induced Somatic Sector Analysis ISSA for Studying Genes and Promoters Involved in Wood Formation and Secondary Stem Development

Published on: October 5, 2016

9.3K

Characterization of DWARF14 Genes in Populus.

Kaijie Zheng1,2, Xiaoping Wang1,2, Deborah A Weighill1,3

  • 1Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831, USA.

Scientific Reports
|February 16, 2016
PubMed
Summary

Researchers identified two DWARF14 (D14) homologs in poplar trees, finding that PtD14a functions as the key strigolactone receptor. This discovery is crucial for understanding plant hormone regulation in woody plants.

More Related Videos

Poplar Adventitious Roots Induced by Stem Canker Pathogens: An Experimental System for Studying Roots Biology and Light Response-Related Processes
08:04

Poplar Adventitious Roots Induced by Stem Canker Pathogens: An Experimental System for Studying Roots Biology and Light Response-Related Processes

Published on: October 11, 2024

562
CcCIPK14 Gene Function Analysis to Illuminate the Efficient Root Transgenic System
07:00

CcCIPK14 Gene Function Analysis to Illuminate the Efficient Root Transgenic System

Published on: September 23, 2021

2.3K

Related Experiment Videos

Last Updated: Mar 25, 2026

The Use of Induced Somatic Sector Analysis ISSA for Studying Genes and Promoters Involved in Wood Formation and Secondary Stem Development
09:54

The Use of Induced Somatic Sector Analysis ISSA for Studying Genes and Promoters Involved in Wood Formation and Secondary Stem Development

Published on: October 5, 2016

9.3K
Poplar Adventitious Roots Induced by Stem Canker Pathogens: An Experimental System for Studying Roots Biology and Light Response-Related Processes
08:04

Poplar Adventitious Roots Induced by Stem Canker Pathogens: An Experimental System for Studying Roots Biology and Light Response-Related Processes

Published on: October 11, 2024

562
CcCIPK14 Gene Function Analysis to Illuminate the Efficient Root Transgenic System
07:00

CcCIPK14 Gene Function Analysis to Illuminate the Efficient Root Transgenic System

Published on: September 23, 2021

2.3K

Area of Science:

  • Plant Biology
  • Molecular Genetics
  • Hormone Signaling

Background:

  • Strigolactones are plant hormones regulating shoot branching and symbiotic interactions.
  • DWARF14 (D14) proteins perceive strigolactone signals in herbaceous plants.
  • Knowledge of D14 genes in woody plants remains limited.

Purpose of the Study:

  • Identify and characterize D14 homologs in the model woody plant, Populus trichocarpa (poplar).
  • Determine the functional role of identified poplar D14 homologs in strigolactone signaling.

Main Methods:

  • Bioinformatic analysis to identify D14 homologs in Populus trichocarpa.
  • Gene expression analysis using quantitative PCR.
  • Complementation assays in Arabidopsis d14 mutants.
  • Amino acid alignment and protein structural modeling.

Main Results:

  • Two D14 homologs, PtD14a and PtD14b, were identified in P. trichocarpa with high amino acid similarity (>95%).
  • PtD14a transcript levels were generally higher than PtD14b.
  • Only PtD14a successfully complemented Arabidopsis d14 mutants, indicating it is the functional ortholog.
  • PtD14b exhibited amino acid substitutions in conserved regions, potentially affecting its function.

Conclusions:

  • PtD14a is identified as the functional DWARF14 ortholog in Populus trichocarpa.
  • Structural differences in PtD14b may explain its lack of function.
  • This study provides a foundation for investigating strigolactone pathways in woody perennial plants.