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Updated: Mar 25, 2026
Digital PCR for Quantifying Circulating MicroRNAs in Acute Myocardial Infarction and Cardiovascular Disease
Published on: July 3, 2018
Plasma microRNA-21 is a potential diagnostic biomarker of acute myocardial infarction
1Department of Cardiology, Tianjin Chest Hospital, Tianjin, China. yujieliu2012@126.com.
Objective:
Previous studies have demonstrated that microRNA-21 (miR-21) is involved in the pathogenesis of myocardium infarction and cardiac fibrosis; the present study aimed to investigate its potential role in the diagnosis of acute myocardium infarction (AMI).
Patients And Methods:
A cohort of patients with AMI and angina pectoris (AP) were studied, plasma miR-21 level was determined by Realtime-PCR.
Results:
We found that the plasma miR-21 level was significantly elevated in patients with AMI compared with those with AP or healthy people. Further studies demonstrated the correlation of miR-21 and several traditional markers such as creatine kinase (CK), creatine kinase-MB (CK-MB) and troponin I (cTnI) in study subjects. Finally, receiver-operator characteristic curve (ROC) analysis showed that miR-21 has similar diagnostic ability compared with CK, CK-MB and cTnI.
Conclusions:
Plasma miR-21 may be a novel biomarker for the diagnosis of AMI. Our study may also provide implications for the development of new biomarkers.
Insights
Plasma microRNA-21 (miR-21) levels are significantly elevated in patients with acute myocardial infarction (AMI). This finding suggests miR-21 may serve as a novel biomarker for diagnosing AMI.
Area of Science:
- Biochemistry
- Molecular Biology
- Cardiology
Background:
- MicroRNA-21 (miR-21) is implicated in myocardial infarction and cardiac fibrosis.
- The diagnostic potential of miR-21 in acute myocardial infarction (AMI) requires further investigation.
Purpose of the Study:
- To evaluate the role of plasma miR-21 as a diagnostic biomarker for AMI.
- To compare the diagnostic ability of miR-21 with traditional cardiac markers.
Main Methods:
- Plasma miR-21 levels were quantified using Real-Time PCR in patients with AMI and angina pectoris (AP).
- Correlations between miR-21 and cardiac markers (creatine kinase, CK-MB, troponin I) were assessed.
- Receiver-operator characteristic (ROC) curve analysis was performed.
Main Results:
- Plasma miR-21 levels were significantly higher in AMI patients compared to AP patients and healthy individuals.
- miR-21 showed a positive correlation with traditional cardiac markers.
- ROC analysis indicated that miR-21 possesses diagnostic capabilities comparable to CK, CK-MB, and cTnI.
Conclusions:
- Plasma miR-21 emerges as a potential novel biomarker for the diagnosis of AMI.
- This research may guide the development of innovative diagnostic tools for cardiac conditions.
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