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Complete genome sequence of mumps viruses isolated from patients with parotitis, pancreatitis and encephalitis in
Sunil R Vaidya1, Deepika T Chowdhury1, Santoshkumar M Jadhav1
1National Institute of Virology, Indian Council of Medical Research, 20-A Dr Ambedkar Road, Pune 411001, India.
Abstract:
Limited information is available regarding epidemiology of mumps in India. Mumps vaccine is not included in the Universal Immunization Program of India. The complete genome sequences of Indian mumps virus (MuV) isolates are not available, hence this study was performed. Five isolates from bilateral parotitis and pancreatitis patients from Maharashtra, a MuV isolate from unilateral parotitis patient from Tamil Nadu, and a MuV isolate from encephalitis patient from Uttar Pradesh were genotyped by the standard protocol of the World Health Organization and subsequently complete genomes were sequenced. Indian MuV genomes were compared with published MuV genomes, including reference genotypes and eight vaccine strains for the genetic differences. The SH gene analysis revealed that five MuV isolates belonged to genotype C and two belonged to genotype G strains. The percent nucleotide divergence (PND) was 1.1% amongst five MuV genotype C strains and 2.2% amongst two MuV genotype G strains. A comparison with widely used mumps Jeryl Lynn vaccine strain revealed that Indian mumps isolates had 54, 54, 53, 49, 49, 38, and 49 amino acid substitutions in Chennai-2012, Kushinagar-2013, Pune-2008, Osmanabad-2012a, Osmanabad-2012b, Pune-1986 and Pune-2012, respectively. This study reports the complete genome sequences of Indian MuV strains obtained in years 1986, 2008, 2012 and 2013 that may be useful for further studies in India and globally.
Insights
This study sequenced complete mumps virus (MuV) genomes from India, revealing genetic differences from vaccine strains. These findings are crucial for understanding mumps epidemiology and vaccine effectiveness in the region.
Area of Science:
- Virology
- Epidemiology
- Genomics
Background:
- Limited epidemiological data exists for mumps in India.
- The mumps vaccine is not part of India's Universal Immunization Program.
- Complete genome sequences of Indian mumps virus (MuV) isolates were previously unavailable.
Purpose of the Study:
- To sequence and analyze complete Indian MuV genomes.
- To compare Indian MuV strains with global reference genotypes and vaccine strains.
- To identify genetic differences relevant to mumps epidemiology in India.
Main Methods:
- Whole genome sequencing of seven MuV isolates from parotitis, pancreatitis, and encephalitis patients.
- Genotyping using the World Health Organization standard protocol.
- Phylogenetic analysis and comparison of nucleotide and amino acid sequences with reference strains.
Main Results:
- Five MuV isolates belonged to genotype C and two to genotype G.
- Percent nucleotide divergence was 1.1% within genotype C and 2.2% within genotype G strains.
- Significant amino acid substitutions were observed when compared to the Jeryl Lynn vaccine strain.
Conclusions:
- This study provides the first complete genome sequences of Indian MuV strains from various years (1986-2013).
- Identified genetic variations may impact mumps vaccine efficacy and disease surveillance in India.
- The data is valuable for future research on mumps virus evolution and control strategies in India and globally.
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