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Interactive mechanism between avian infectious bronchitis S1 protein T cell peptide and avian MHC I molecule

Feng-zhu Zhu1, Mei Lu2, Qing-hua Huang3

  • 1Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Shandong Key Lab of Animal Disease Control and Breeding, Jinan, Shandong 2501001, PR China; College of Animal Science, Shandong Agricultural University, Tai'an, Shandong 271018, PR China.

Virus Research
|February 16, 2016
PubMed

This study aims to construct a 3D structure of the avian major histocompatibility complex (MHC)-β2M complex through homology modelling technology, perform molecular docking of the predicted infectious bronchitis virus (IBV) S1 protein potential epitope peptide Sp6 (NQFYIKLT) and the avian MHC-β2M complex, and demonstrate the interactive mechanism between Sp6 and MHC using molecular dynamical simulations. The peptide Sp6 and the non-related peptide NP89-97 (PKKTGGPIY) were used to stimulate in vitro recombinant plasmid (pCAGGS-S1) avian splenic lymphocytes. Flow cytometric results show that CD8(+) T lymphocytes reproduce stimulated by the Sp6 and the nonrelated peptide proliferate by 34.8% and 2.6%, respectively. Meanwhile, fluorescent quantitative PCR results show that the secretion of IFN-γ in avian splenic lymphocytes increases after Sp6 stimulation. These data suggest that Sp6 can induce the activated avian lymphocytes in vitro to produce CTL, which is the CTL epitope in IBV S1.

Keywords:
Avian infectious bronchitis virusCTL epitopeHomology modelling

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