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Phosphodiesterase 6β Expression In Developing Mouse Retina.

Fadi Assaf1, Ju Zhang1, Judith Mosinger Ogilvie1

  • 1Department of Biology, Saint Louis University, St. Louis, Missouri 63103.

Impulse (Columbia, S.C.)
|February 16, 2016
PubMed
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Phosphodiesterase 6β (PDE6β) protein is detected early in wild type mouse retinal development but absent in the rd1 model. PDE6β and PRA1 do not co-localize, suggesting PRA1

Area of Science:

  • Retinal biology
  • Developmental neuroscience
  • Molecular ophthalmology

Background:

  • The rd1 mouse model exhibits retinitis pigmentosa (RP) due to a Pde6b gene mutation, impacting phototransduction.
  • Pde6b gene expression is embryonic, preceding other phototransduction proteins expressed around postnatal day 5 (P5).
  • Prenylated Rab Acceptor 1 (PRA1), a vesicular trafficking protein, is downregulated in the developing rd1 retina.

Purpose of the Study:

  • To investigate the temporal and spatial expression of Phosphodiesterase 6β (PDE6β) protein during mouse photoreceptor development.
  • To explore the potential relationship and co-localization between PDE6β and PRA1 in the developing retina.

Main Methods:

  • Western blotting of wild type and rd1 mouse retinas from postnatal day 2 (P2) to P21.
Keywords:
PDE6βPRA1cGMPdevelopmentmousephotoreceptorretinal degenerationtrafficking

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  • Immunohistochemistry to detect PDE6β and PRA1 expression and localization during postnatal development.

    • Main Results:

    • PDE6β protein is detectable in wild type retinas by P2 and absent in rd1 retinas.
    • Earliest PDE6β detection by immunohistochemistry was at P6, localized to the apical photoreceptor layer.
    • No co-localization was observed between PDE6β and PRA1 during postnatal differentiation.

    Conclusions:

    • PDE6β expression precedes photoreceptor differentiation and other phototransduction proteins, suggesting a potential developmental role beyond phototransduction.
    • The lack of co-localization indicates no direct interaction between PDE6β and PRA1 during the studied developmental period.
    • The precise function of PRA1 in retinal development remains undetermined.