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Updated: Mar 25, 2026

Techniques for the Evolution of Robust Pentose-fermenting Yeast for Bioconversion of Lignocellulose to Ethanol

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Published on: October 24, 2016

Improving cellulase production by Aspergillus niger using adaptive evolution.

Aleksandrina Patyshakuliyeva¹, Mark Arentshorst², Iris E Allijn¹

¹Fungal Molecular Physiology, CBS-KNAW Fungal Biodiversity Centre, Utrecht University, Utrecht, The Netherlands.

Biotechnology Letters

|February 17, 2016

Summary

This summary is machine-generated.

Adaptive evolution efficiently enhanced cellulose production in Aspergillus niger by fivefold. This method activated genes for polysaccharide degradation, highlighting its potential for industrial enzyme production.

Keywords:

Adaptive evolution Aspergillus niger Cellulose NADPH oxidase NoxR

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Area of Science:

Microbiology
Biotechnology
Enzyme Engineering

Background:

Enzyme production is crucial for plant biomass degradation.
Adaptive evolution offers a potential strategy for strain improvement.

Purpose of the Study:

To assess adaptive evolution for enhancing plant biomass degrading enzyme production.
To identify genetic modifications underlying improved enzyme yield.

Main Methods:

Adaptive evolution of Aspergillus niger.
Physiological characterization of evolved strains.
Transcriptomic analysis and gene knockout studies.

Main Results:

An Aspergillus niger mutant with fivefold higher cellulose production was generated.
Reduced expression of noxR (NADPH oxidase complex regulatory subunit) was observed.
Knockout of noxR confirmed its role in enhanced cellulose degradation.

Conclusions:

Adaptive evolution is effective for strain modification.
This approach can activate genes involved in polysaccharide degradation.