Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

First report of tomato brown rugose fruit virus infecting <i>Solanum lycopersicum</i> in Northeast China.

Plant disease·2023
Same author

Identification of P3H1 as a Predictive Prognostic Biomarker for Bladder Urothelial Carcinoma Based on the Cancer Genome Atlas Database.

Pharmacogenomics and personalized medicine·2023
Same author

Lipid-anchored proteasomes control membrane protein homeostasis.

Science advances·2023
Same author

IN VITRO EVALUATION OF A NOVEL AUTOMATIC INTRAOPERATIVE BLOOD LOSS MONITOR.

Shock (Augusta, Ga.)·2023
Same author

A multiplex Taqman PCR assay for MRSA detection from whole blood.

PloS one·2023
Same author

Spinal HDAC6 mediates nociceptive behaviors induced by chronic constriction injury via neuronal activation and neuroinflammation.

Molecular pain·2023

Related Experiment Video

Updated: Mar 25, 2026

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis
14:53

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

Published on: September 10, 2014

17.9K

Microfluidic chip for stacking, separation and extraction of multiple DNA fragments.

Ruige Wu1, Y P Seah1, Zhiping Wang1

  • 1Singapore Institute of Manufacturing Technology, 71 Nanyang drive, 638075, Singapore.

Journal of Chromatography. A
|February 17, 2016
PubMed
Summary

A novel microfluidic device enables rapid DNA fragment separation and extraction using isotachophoresis hyphenated gel electrophoresis (ITP-GE). This integrated system significantly accelerates DNA analysis, offering a faster alternative to traditional methods.

Keywords:
ExtractionGel electrophoresisIsotachophoresisMicrofluidicSeparation

More Related Videos

A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice
11:32

A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice

Published on: November 23, 2015

14.5K
Microfluidic Applications for Disposable Diagnostics
10:21

Microfluidic Applications for Disposable Diagnostics

Published on: February 3, 2008

9.4K

Related Experiment Videos

Last Updated: Mar 25, 2026

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis
14:53

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

Published on: September 10, 2014

17.9K
A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice
11:32

A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice

Published on: November 23, 2015

14.5K
Microfluidic Applications for Disposable Diagnostics
10:21

Microfluidic Applications for Disposable Diagnostics

Published on: February 3, 2008

9.4K

Area of Science:

  • Biotechnology
  • Analytical Chemistry
  • Molecular Biology

Background:

  • Traditional DNA fragment analysis methods are time-consuming and labor-intensive.
  • Need for rapid, efficient, and integrated solutions for DNA extraction and purification.

Purpose of the Study:

  • To develop a disposable integrated microfluidic device for rapid DNA fragment analysis.
  • To achieve simultaneous pre-concentration, separation, and on-chip extraction of DNA fragments.

Main Methods:

  • Isotachophoresis hyphenated gel electrophoresis (ITP-GE) for pre-concentration and separation.
  • On-chip electroelution for DNA fragment extraction.
  • Integration of these techniques onto a single microfluidic chip.

Main Results:

  • Successful separation and extraction of 200bp, 500bp, and 1kbp DNA fragments within 25 minutes.
  • Extraction efficiencies of 49.9%, 52.1%, and 53.7% for the respective DNA fragments.
  • Demonstrated compatibility of extracted DNA with downstream enzymatic reactions like PCR.
  • Comparable performance to traditional gel cutting for sheared genomic DNA extraction.
  • Overall assay time reduced to 32 minutes, six times faster than conventional methods.

Conclusions:

  • The developed microfluidic device offers a rapid and efficient integrated solution for DNA fragment analysis.
  • This technology significantly reduces assay time and maintains DNA integrity for downstream applications.
  • The disposable integrated chip presents a promising alternative to traditional DNA extraction methods.