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Related Experiment Videos

Viruses with bisegmented double-stranded RNA in pig faeces.

M S Gatti1, A F de Castro, M M Ferraz

  • 1Department of Microbiology and Immunology, Campinas State University, Brazil.

Research in Veterinary Science
|November 1, 1989
PubMed
Summary

New bisegmented double-stranded (ds) RNA viruses were found in Brazilian pigs. These viruses, similar to picobirnaviruses, were detected in 15.3% of pigs with diarrhea, suggesting a potential link to the illness.

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Area of Science:

  • Virology
  • Molecular Biology
  • Veterinary Medicine

Background:

  • Bisegmented double-stranded (ds) RNA viruses, including picobirnaviruses, have been identified in various animal species.
  • Previous studies have documented picobirnaviruses in human feces and the intestinal contents of rodents and guinea pigs.
  • The presence and potential impact of similar viruses in swine populations remained largely unexplored.

Purpose of the Study:

  • To investigate the presence of bisegmented dsRNA viruses in fecal samples from pigs in Sao Paulo, Brazil.
  • To determine if there is an association between these viruses and diarrhea in pigs.
  • To characterize the detected viruses using molecular techniques.

Main Methods:

  • Fecal samples were collected from 912 pigs of various breeds and ages (9-61 days) in Sao Paulo, Brazil.

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  • Samples were analyzed using polyacrylamide gel electrophoresis with silver staining to detect dsRNA.
  • A combined enzyme immunoassay for rotavirus and adenovirus was performed, with simian rotavirus SA11 as a control.

    • Main Results:

    • Electrophoretic profiles indicative of bisegmented dsRNA viruses were detected in 106 out of 912 pigs (11.6% prevalence).
    • The prevalence was higher in pigs with diarrhea (15.3%) compared to pigs without diarrhea (9.6%).
    • These findings suggest a potential association between bisegmented dsRNA viruses and enteric disease in pigs.

    Conclusions:

    • Bisegmented dsRNA viruses, similar to picobirnaviruses, are present in the pig population of Sao Paulo, Brazil.
    • The increased detection rate in diarrheic animals suggests these viruses may play a role in swine enteric disease.
    • Further research is warranted to fully characterize these viruses and confirm their etiological role in piglet diarrhea.