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10:34
A High-content Assay for Monitoring AMPA Receptor Trafficking
Published on: January 28, 2019
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Transferrin Receptor Controls AMPA Receptor Trafficking Efficiency and Synaptic Plasticity
Ke Liu1,2,3,4, Run Lei1,2,3,4, Qiong Li5
1West China Developmental &Stem Cell Institute, West China Second Hospital, State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, Chengdu, Sichuan 610041, China.
Scientific Reports
|February 17, 2016
Summary
Transferrin receptor (TFR) regulates AMPA receptor trafficking and synaptic plasticity. Loss of TFR in neural progenitor cells leads to epilepsy and impaired synaptic function in mice.
Area of Science:
- Neuroscience
- Cell Biology
- Iron Metabolism
Background:
- Transferrin receptor (TFR) is crucial for iron transport and clathrin-mediated endocytosis.
- Its role beyond iron homeostasis in central nervous system (CNS) development is largely unexplored.
Purpose of the Study:
- To investigate the non-iron transport functions of TFR in CNS development.
- To elucidate TFR's role in regulating AMPA receptor trafficking and synaptic plasticity.
Main Methods:
- Conditional knockout (KO) of TFR in neural progenitor cells of mice.
- Assessment of synaptic transmission, long-term potentiation (LTP), and AMPA receptor dynamics.
Main Results:
- TFR KO mice exhibited progressive epileptic seizures.
- Basal synaptic transmission and LTP were significantly reduced in TFR KO mice.
- TFR KO decreased GluR2 binding to AP2, impairing AMPA receptor endocytosis and recycling.
Conclusions:
- TFR acts as a novel regulator of AMPA receptor trafficking efficiency.
- TFR is essential for maintaining synaptic plasticity and normal brain function.
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