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Updated: Mar 25, 2026
Real-time Cytotoxicity Assays in Human Whole Blood
Published on: November 7, 2014
Doxorubicin enhances (131)I-rituximab induced cell death in Raji cells
C Kumar, B N Pandey, G Samuel1
1Isotope Applications and Radiopharmaceuticals Division, Bhabha Atomic Research Centre, Mumbai, Maharashtra, India.
Aim:
There are various therapeutic modalities of treatment for non-Hodgkin's lymphoma, but with certain limitations, hence, investigating the scope of combined therapeutic approach.
Materials And Methods:
In this article, cellular toxicity, apoptosis and expression of mitogen-activated protein kinase signaling pathway proteins were investigated in Raji cells preincubated with doxorubicin followed by (131)I-rituximab (rituximab radiolabeled with Iodine-131) treatment.
Results:
It was found that the (131)I-rituximab in combination with doxorubicin showed a higher amount of cell toxicity and apoptosis compared to respective controls. Expression of anti-apoptotic protein (B-cell lymphoma-extra-large) was downregulated and cleavage of poly (ADP-ribose) polymerase, a marker of apoptosis was higher in cells treated with doxorubicin (2 μg/mL) and 131 I-rituximab (P ≤ 0.05). Moreover, in these cells the basal level of expression of p42/44 and p38 were increased while its phosphorylation was decreased.
Conclusion:
These results suggest that doxorubicin has the potential to sensitize (131)I-rituximab induced cell death in Raji cells.
