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Related Concept Videos

Regulation of Hematopoietic Stem Cells01:01

Regulation of Hematopoietic Stem Cells

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All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...
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Multipotency of Hematopoietic Stem Cells01:19

Multipotency of Hematopoietic Stem Cells

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The hematopoietic stem cells or HSCs are multipotent, meaning they can differentiate and give rise to all blood and immune cells. HSCs are maintained in the quiescent stage until an external stimulus initiates their differentiation. The multipotent HSCs exist as two heterogeneous populations, long-term repopulating cells (LTRC) and short-term repopulating cells (STRC). The two HSC populations have different surface markers or receptors and are classified based on quiescence and long-term...
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Role of Hematopoietic Growth Factors01:28

Role of Hematopoietic Growth Factors

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Hematopoietic growth factors are molecules that regulate the differentiation rate of hematopoietic stem cells (HSCs). Erythropoietin (EPO), primarily produced by the kidneys, plays a crucial role in erythrocyte production. When oxygen levels in the blood are low, EPO is released into the bloodstream, reaching the bone marrow, where it stimulates HSCs to differentiate and mature into erythrocytes, which are vital for oxygen transport.
Thrombopoietin (TPO), mainly released by the liver,...
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Hematopoiesis01:21

Hematopoiesis

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The process of blood cell formation is called hematopoiesis. Hematopoiesis starts early during development, on the seventh day of embryogenesis. This phase of hematopoiesis is called the primitive wave, wherein the extraembryonic yolk sac allows the production of erythroid cells and endothelial cells from a common precursor called hemangioblast. The erythroid cells provide oxygen to support the growth of the rapidly dividing embryo. Hemangioblasts later develop into hematopoietic stem cells or...
9.7K
Overview of Hematopoiesis01:20

Overview of Hematopoiesis

11.3K
Hematopoiesis, or blood cell production, is a vital biological process that begins early in embryonic development and continues throughout life. This process generates the various types of cells found in blood, including red blood cells, white blood cells, and platelets from hematopoietic stem cells (HSCs).
Developmental Phases of Hematopoiesis
Initially, HSCs are formed in the embryonic yolk sac, a critical site for early blood cell production. These stem cells subsequently migrate to other...
11.3K
Production of Formed Elements01:34

Production of Formed Elements

5.3K
Hemangioblasts are multipotent stem cells originating from the mesoderm. They give rise to hematopoietic stem cells (HSCs), which undergo hematopoiesis to produce all the formed elements of blood. This process is regulated by a complex network of hematopoietic growth factors, including transcription factors, growth factors, and cytokines. These factors stimulate the HSCs to divide and differentiate, though some HSCs remain undifferentiated to maintain a self-renewing pool.
Most HSCs commit to...
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Related Experiment Video

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Phenotypic Analysis and Isolation of Murine Hematopoietic Stem Cells and Lineage-committed Progenitors
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Hematopoietic stem cells: multiparameter regulation.

Kedong Song1, Liying Li2, Yiwei Wang3

  • 1State Key Laboratory of Fine Chemicals, Dalian R&D Center for Stem Cell and Tissue Engineering, Dalian University of Technology, Dalian, 116024, China. kedongsong@dlut.edu.cn.

Human Cell
|February 18, 2016
PubMed
Summary
This summary is machine-generated.

Hematopoietic stem cells (HSCs) hold therapeutic promise but are limited by insufficient numbers. This review explores methods like growth factors and biomaterials to improve in vitro HSC expansion for clinical use.

Keywords:
Ex vivo expansionHematopoietic stem cells (HSCs)Multiparameter regulation

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Flow Cytometry Analysis of Murine Bone Marrow Hematopoietic Stem and Progenitor Cells and Stromal Niche Cells
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Analysis of Hematopoietic Stem Progenitor Cell Metabolism
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Analysis of Hematopoietic Stem Progenitor Cell Metabolism

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Area of Science:

  • * Regenerative Medicine
  • * Stem Cell Biology
  • * Hematopoiesis

Background:

  • * Hematopoietic stem cells (HSCs) possess self-renewal and multi-potency, offering significant clinical therapy potential.
  • * A primary limitation for HSC clinical application is the insufficient number of cells obtained from bone marrow, peripheral blood, or umbilical cord blood.
  • * Optimizing HSC expansion is crucial for advancing cell-based therapies.

Purpose of the Study:

  • * To review the key factors influencing hematopoietic stem cell expansion in vitro.
  • * To provide a comprehensive understanding of strategies for enhancing HSC numbers for clinical applications.
  • * To discuss the role of microenvironmental components in controlling hematopoiesis.

Main Methods:

  • * Review of literature on growth factors, cytokines, and stromal cell support for HSCs.
  • * Analysis of the impact of extracellular matrix and bionic scaffolds on HSC behavior.
  • * Examination of the role of the microenvironment in regulating hematopoiesis.
  • * Synthesis of current knowledge on in vitro HSC expansion techniques.

Main Results:

  • * Growth factors and cytokines are essential for HSC self-renewal and differentiation.
  • * Stromal cells and extracellular matrix provide critical supportive cues for HSC maintenance.
  • * Bionic scaffolds can mimic the natural niche to promote HSC expansion.
  • * Understanding the interplay of these components is key to controlling hematopoiesis in vitro.

Conclusions:

  • * In vitro expansion of hematopoietic stem cells is feasible through careful manipulation of the cellular and material environment.
  • * The combination of biological factors (growth factors, cells) and biomaterials (scaffolds) offers a promising avenue for generating sufficient HSCs for clinical use.
  • * Further research into optimizing the in vitro microenvironment will enhance the therapeutic application of HSCs.