Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Overview of Cell-Matrix Interactions

Overview of Cell-Matrix Interactions

The extracellular matrix or ECM holds cells together to form a tissue and allows the cells within the tissue to communicate. ECM comprises proteins such as fibronectin, collagen, laminin, etc. The most abundant protein in this space is collagen. Collagen fibers are interwoven with carbohydrate-containing protein molecules called proteoglycans. ECM allows cell migration and provides a structural scaffold at cell adhesion that anchors the cell when the extracellular matrix proteins interact with...

Cell-matrix's Response to Mechanical Forces

Cell-matrix's Response to Mechanical Forces

In animal cells, the extracellular matrix allows cells within tissues to withstand external stresses and transmits signals from the outside of the cell to the inside. The extracellular matrix is extensive, and its composition varies between different types of tissues. For example, the reticular fibers and ground substance make up the ECM in loose connective tissue, while collagen and bone minerals make up the ECM of bone tissue.
Anchoring junctions mechanically attach a cell to the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

First Measurement of Time-Dependent CP Violation in the Flavor-Changing Neutral-Current Decay B^{0}→K_{S}^{0}μ^{+}μ^{-}.

Physical review letters·2026

Same author

Measurement of the Top-Quark Production Cross Section and Charge Asymmetry at LHCb.

Physical review letters·2026

Same author

Searches for B^{0}→K^{+}π^{-}τ^{+}τ^{-} and B_{s}^{0}→K^{+}K^{-}τ^{+}τ^{-} Decays.

Physical review letters·2026

Same author

First Evidence of the B_{s}^{0}→K^{-}π^{+}γ Decay.

Physical review letters·2026

Same author

Precision Measurement of CP Violation and Branching Fractions in B^{±}→K_{S}^{0}h^{±} (h=π, K) Decays and Search for the Rare Decay B_{c}^{±}→K_{S}^{0}K^{±}.

Physical review letters·2026

Same author

First Observation of the B[over ¯]_{s}^{0}→Λ_{c}^{+}Λ[over ¯]_{c}^{-} Decay and Evidence for the B[over ¯]^{0}→Λ_{c}^{+}Λ[over ¯]_{c}^{-} Decay.

Physical review letters·2026

Search research articles

Related Experiment Video

Updated: Mar 25, 2026

A High-throughput Cell Microarray Platform for Correlative Analysis of Cell Differentiation and Traction Forces

A High-throughput Cell Microarray Platform for Correlative Analysis of Cell Differentiation and Traction Forces

Published on: March 1, 2017

Extracellular matrix microarrays to study inductive signaling for endoderm specification.

D F Braga Malta¹, N E Reticker-Flynn², C L da Silva³

¹Massachusetts Institute of Technology, Cambridge, MA, United States; Department of Bioengineering and IBB-Institute for Bioengineering and Biosciences, Instituto Superior Técnico, Universidade de Lisboa, Portugal.

Acta Biomaterialia

|February 18, 2016

Summary

Researchers developed a high-throughput platform to study extracellular matrix (ECM) effects on stem cell differentiation. This approach identified specific ECM combinations that guide endoderm progenitor cells toward liver and pancreas fates, improving differentiation protocols.

Keywords:

Extracellular matrix High-throughput Liver Microarray Pancreas

More Related Videos

Fabrication and Use of MicroEnvironment microArrays MEArrays

Fabrication and Use of MicroEnvironment microArrays MEArrays

Published on: October 11, 2012

Engineering Three-dimensional Epithelial Tissues Embedded within Extracellular Matrix

Engineering Three-dimensional Epithelial Tissues Embedded within Extracellular Matrix

Published on: July 10, 2016

Related Experiment Videos

Last Updated: Mar 25, 2026

A High-throughput Cell Microarray Platform for Correlative Analysis of Cell Differentiation and Traction Forces

A High-throughput Cell Microarray Platform for Correlative Analysis of Cell Differentiation and Traction Forces

Published on: March 1, 2017

Fabrication and Use of MicroEnvironment microArrays MEArrays

Fabrication and Use of MicroEnvironment microArrays MEArrays

Published on: October 11, 2012

Engineering Three-dimensional Epithelial Tissues Embedded within Extracellular Matrix

Engineering Three-dimensional Epithelial Tissues Embedded within Extracellular Matrix

Published on: July 10, 2016

Area of Science:

Stem cell biology
Developmental biology
Biomaterials science

Background:

Microenvironmental cues critically regulate stem and progenitor cell fate decisions during tissue development.
Current methods limit systematic exploration of complex extracellular matrix (ECM) interactions at scale.
Understanding ECM's role is vital for improving stem cell differentiation protocols.

Purpose of the Study:

To develop and utilize a high-throughput extracellular matrix (ECM) array platform to systematically investigate ECM's influence on endoderm progenitor cell differentiation.
To identify specific ECM combinations that direct definitive endoderm progenitors towards hepatic and pancreatic lineages.
To explore ECM-mediated modifications of signaling pathways during liver specification.

Main Methods:

Implementation of a high-throughput ECM array platform testing 741 distinct combinations of 38 ECM components.
Use of embryonic stem cell-derived definitive endoderm progenitors.
Analysis of ECM influence on lineage fate, cell adhesion, survival, and signaling pathway activation (e.g., SMAD pathway).

Main Results:

Identification of specific ECM combinations, including fibronectin and laminin isoforms and collagen subtypes, that significantly influence endoderm fate decisions.
Demonstration that defined ECM compositions modulate SMAD pathway activation during hepatic differentiation.
Evidence that ECM components impact lineage choice, cell adhesion, and survival during differentiation.

Conclusions:

High-throughput cellular microarrays are powerful tools for investigating microenvironmental signals in cell function and fate regulation.
This study validates the utility of a cellular microarray for analyzing ECM effects on liver and pancreas differentiation of endoderm progenitors.
The platform's broad applicability extends to investigating cell fate regulation in other developmental and disease contexts.