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Related Experiment Video

Updated: Mar 25, 2026

A Simple High Efficiency Protocol for Pancreatic Islet Isolation from Mice
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A Simple High Efficiency Protocol for Pancreatic Islet Isolation from Mice

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Experimental studies on islets isolation, purification and function in rats.

Xinlu Pang1, Wujun Xue2, Xinshun Feng2

  • 1Department of Kidney Transplantation, The First Affiliated Hospital of Zhengzhou University Zhengzhou 450052, China.

International Journal of Clinical and Experimental Medicine
|February 18, 2016
PubMed
Summary
This summary is machine-generated.

This study presents an effective rat islet isolation method using collagenase P injection and gravity gradient purification. The developed technique yields highly viable and functional islets, successfully restoring normal blood sugar levels in diabetic rats post-transplantation.

Keywords:
Isletisolationpurification

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Area of Science:

  • Endocrinology
  • Cell Biology
  • Surgical Techniques

Background:

  • Islet isolation is crucial for diabetes research and transplantation.
  • Existing methods can be complex and yield variable results.

Purpose of the Study:

  • To establish a simple and effective rat islet isolation and purification protocol.
  • To assess the viability and function of isolated islets.

Main Methods:

  • Rat pancreas digestion via collagenase P injection into the pancreatic duct.
  • Incubation in a water bath for tissue digestion.
  • Discontinuous gravity gradient purification for islet isolation.
  • Dithizone staining for islet identification.
  • Acridine orange (AO) and propidium iodide (PI) fluorescence staining for viability assessment.
  • Glucose-stimulated insulin release assay and transplantation into diabetic rats for functional evaluation.

Main Results:

  • Recovery of 738±193 islets per rat with an average purity of 77±13%.
  • Islet viability exceeded 95%.
  • Demonstrated significant glucose-stimulated insulin release (low glucose: 24.31±5.47 mIU/L; high glucose: 37.62±4.29 mIU/L).
  • Successful restoration of normal blood glucose levels in diabetic rats within two days post-transplantation.

Conclusions:

  • The described method provides a simple, effective, and reproducible approach for rat islet isolation and purification.
  • Isolated islets exhibit excellent viability, morphology, and function.
  • This technique holds promise for advancing diabetes research and therapeutic strategies.